PMID- 19285752
OWN - NLM
STAT- MEDLINE
DCOM- 20090724
LR  - 20200930
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 166
IP  - 10
DP  - 2009 Jul 1
TI  - Epigenetic changes detected in micropropagated hop plants.
PG  - 1101-11
LID - 10.1016/j.jplph.2008.12.015 [doi]
AB  - Micropropagation is a widely used technique in hops (Humulus lupulus L.). 
      However, to the best of our knowledge, the genetic and epigenetic stability of 
      the microplants has never been tested before. In the present study, two hop 
      accessions were established in vitro and micropropagated for 2 years. The genetic 
      and epigenetic stability of the in vitro plants was analyzed with several 
      molecular techniques: random amplified DNA polymorphism (RAPD), retrotransposon 
      microsatellite amplified polymorphism (REMAP), and methylation-sensitive 
      amplification polymorphism (MSAP). No genetic variation among control and treated 
      plants was found, even after 12 cycles of micropropagation. Epigenetic variation 
      was detected, first, when field and in vitro samples were compared. Nearly a 30% 
      of the detected fragments presented the same pattern of alterations in all the 
      vitroplants. Second, lower levels of epigenetic variation were detected among 
      plants from the different subcultures. Part of this detected variation seemed to 
      be accumulated along the 12 sequential subcultures tested.
FAU - Peredo, Elena L
AU  - Peredo EL
AD  - Universidad de Oviedo, Department of Plant Physiology, Catedratico Rodrigo Uria 
      s/n, 33071 Oviedo, Spain. elperedo.uo@uniovi.es
FAU - Arroyo-Garcia, Rosa
AU  - Arroyo-Garcia R
FAU - Revilla, M Angeles
AU  - Revilla MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090313
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
SB  - IM
MH  - Epigenesis, Genetic/*genetics
MH  - Humulus/*genetics/*growth & development
MH  - Microsatellite Repeats/genetics
MH  - Polymorphism, Genetic/genetics
EDAT- 2009/03/17 09:00
MHDA- 2009/07/25 09:00
CRDT- 2009/03/17 09:00
PHST- 2008/09/25 00:00 [received]
PHST- 2008/11/26 00:00 [revised]
PHST- 2008/12/19 00:00 [accepted]
PHST- 2009/03/17 09:00 [entrez]
PHST- 2009/03/17 09:00 [pubmed]
PHST- 2009/07/25 09:00 [medline]
AID - S0176-1617(09)00045-5 [pii]
AID - 10.1016/j.jplph.2008.12.015 [doi]
PST - ppublish
SO  - J Plant Physiol. 2009 Jul 1;166(10):1101-11. doi: 10.1016/j.jplph.2008.12.015. 
      Epub 2009 Mar 13.