PMID- 19288023
OWN - NLM
STAT- MEDLINE
DCOM- 20090807
LR  - 20190606
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 23
IP  - 4
DP  - 2009 Apr
TI  - Changes of hepatic proteome in bile duct ligated rats with hepatic fibrosis 
      following treatment with Yin-Chen-Hao-Tang.
PG  - 477-84
AB  - Yin-Chen-Hao-Tang (YCHT) is recognized as a hepatoprotective agent for various 
      types of liver diseases. Proteomics approaches were used to study hepatic and 
      serum protein expression changes in bile duct ligated (BDL) rats following YCHT 
      treatment for 27 days. Two-dimensional gel electrophoresis was used to analyze 
      proteome changes. Of the proteins that exhibited changes, 17 were identified by 
      means of matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) 
      mass spectrometry. The major effect of YCHT was evident in cytoskeleton related 
      protein, plectin-1. In addition, proteins involved in metabolism of lipids were 
      also shown to be affected, including low-density lipoprotein receptor-related 
      protein 2 precursor (glycoprotein 330) and apolipoprotein A-I precursor (ApoA-I). 
      Significant up-regulation of keratin 8 and 19 was found in liver tissue of BDL 
      rats. Supplementation with YCHT also triggered alterations in the above proteins. 
      Interestingly, YCHT treatment caused a statistically significant down-regulation 
      in the secretion of monocyte chemoattractant protein-1 (MCP-1) and tissue 
      inhibitor of metalloproteinase-1 (TIMP-1) in BDL rats with fibrosis. Our results 
      suggested that YCHT may be useful for treatment of liver fibrosis because of its 
      possible antiapoptotic properties, and the therapeutic effects of YCHT on liver 
      diseases might be associated with its lipid biosynthesis regulation.
FAU - Lee, Tzung-Yan
AU  - Lee TY
AD  - Graduate Institute of Traditional Chinese Medicine, School of Chinese Medicine, 
      Chang Gung University, and Center for Traditional Chinese Medicine, Chang Gung 
      Memorial Hospital, Kwei-Shan Taoyuan 333, Taiwan, ROC. joyamen@mail.cgu.edu.tw
FAU - Chang, Hen-Hong
AU  - Chang HH
FAU - Kuo, Jong-Jen
AU  - Kuo JJ
FAU - Shen, Jiann-Jong
AU  - Shen JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Blood Proteins)
RN  - 0 (Drug Combinations)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Proteome)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (yin-chen-hao-tang)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Artemisia/chemistry
MH  - Bile Ducts/surgery
MH  - Blood Proteins/analysis
MH  - Drug Combinations
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gardenia/chemistry
MH  - Gene Expression/drug effects
MH  - Ligation/adverse effects
MH  - Liver/*drug effects/metabolism/pathology
MH  - Liver Cirrhosis/blood/etiology/*prevention & control
MH  - Matrix Metalloproteinase 2/genetics
MH  - Proteome/*analysis
MH  - Proteomics/methods
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rheum/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics
EDAT- 2009/03/17 09:00
MHDA- 2009/08/08 09:00
CRDT- 2009/03/17 09:00
PHST- 2009/03/17 09:00 [entrez]
PHST- 2009/03/17 09:00 [pubmed]
PHST- 2009/08/08 09:00 [medline]
AID - 10.3892/ijmm_00000154 [doi]
PST - ppublish
SO  - Int J Mol Med. 2009 Apr;23(4):477-84. doi: 10.3892/ijmm_00000154.