PMID- 19289584
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20151119
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 131
IP  - 4
DP  - 2009 Apr
TI  - Chromogenic in situ hybridization is a reliable method for detecting HER2 gene 
      status in breast cancer: a multicenter study using conventional scoring criteria 
      and the new ASCO/CAP recommendations.
PG  - 490-7
LID - 10.1309/AJCPI00TVGIGYXAA [doi]
AB  - Chromogenic in situ hybridization (CISH) has shown the potential to replace 
      fluorescence in situ hybridization (FISH) to determine HER2 gene status. To 
      validate the reliability of CISH, we used 226 consecutive breast carcinomas from 
      2 institutions and tested CISH and FISH on the same tumor set simultaneously at 
      different test sites. Besides manufacturers' scoring criteria, the new American 
      Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) 
      guidelines were used to interpret HER2 status. The concordance between CISH and 
      FISH for positive and negative results was 98.5% at site A and 98.6% at site B 
      using the manufacturers' criteria, and 99.0% at site A and 99.1% at site B using 
      the ASCO/CAP criteria. Reproducibility of CISH results was more than 98.0% among 
      3 sites using the manufacturers' criteria and 100.0% between 2 sites using the 
      ASCO/CAP criteria. Our results confirm that CISH is reliable for HER2 testing per 
      ASCO/CAP guidelines.
FAU - Gong, Yun
AU  - Gong Y
AD  - Department of Pathology, The University of Texas M. D. Anderson Cancer Center, 
      1515 Holcombe Blvd, Houston, TX 77030, USA.
FAU - Sweet, William
AU  - Sweet W
FAU - Duh, Yi-Jing
AU  - Duh YJ
FAU - Greenfield, Larry
AU  - Greenfield L
FAU - Fang, Yuan
AU  - Fang Y
FAU - Zhao, Jianxin
AU  - Zhao J
FAU - Tarco, Emily
AU  - Tarco E
FAU - Symmans, W Fraser
AU  - Symmans WF
FAU - Isola, Jorma
AU  - Isola J
FAU - Sneige, Nour
AU  - Sneige N
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Chromogenic Compounds)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Chromogenic Compounds
MH  - Female
MH  - *Genes, erbB-2
MH  - Guidelines as Topic
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
EDAT- 2009/03/18 09:00
MHDA- 2009/04/29 09:00
CRDT- 2009/03/18 09:00
PHST- 2009/03/18 09:00 [entrez]
PHST- 2009/03/18 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
AID - 131/4/490 [pii]
AID - 10.1309/AJCPI00TVGIGYXAA [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2009 Apr;131(4):490-7. doi: 10.1309/AJCPI00TVGIGYXAA.