PMID- 19293472
OWN - NLM
STAT- MEDLINE
DCOM- 20090716
LR  - 20171116
IS  - 1735-1383 (Print)
IS  - 1735-1383 (Linking)
VI  - 6
IP  - 1
DP  - 2009 Mar
TI  - The efficiency of CD40 down regulation by siRNA and antisense ODN: comparison of 
      lipofectamine and FuGENE6.
PG  - 1-11
AB  - BACKGROUND: Dendritic cells (DCs) are ideal accessory cells in the field of gene 
      therapy. Delivery of DNA and siRNA into mammalian cells is a useful technique in 
      treating various diseases caused by single gene defects. Selective gene silencing 
      by small interfering RNAs (siRNAs) and antisense oligodeoxynucleotides (ODN)s is 
      an efficient method for the manipulation of cellular functions. An efficient, 
      functional delivery system with no toxicity problems would be attractive. 
      OBJECTIVE: We compared two commercially available cationic lipids, Lipofectamine 
      and FuGENE6, in the delivery of both siRNA and antisense ODNs into mice 
      spleen-derived DCs. METHODS: Cellular uptake was measured by the means of 
      fluorescein-labelled non-silencing siRNA and antisense ODNs as a model system 
      using flow cytometry. Cytotoxicity of the two delivery systems was compared with 
      propidium iodide and annexin-V staining, and quantified with flow cytometry. The 
      efficiency of our oligonucleotide delivery systems was compared by measuring CD40 
      expression by flow cytometry. RESULTS: CD40 expression in DCs was 38%. After 
      siRNA transfection by Lipofectamine, CD40 expression decreased to 13%, and after 
      transfection by FuGENE6, it decreased to 18%. The difference was statistically 
      significant. CD40 down regulation in DCs transfected with the two different 
      antisense sequences by Lipofectamine was 21% and 23%, and down regulation after 
      transfection by FuGENE6 was 19% and 18%, respectively. The differences were not 
      statistically significant. The effects of siRNA and antisense ODNs were specific. 
      CONCLUSION: Lipofectamine was a more potent delivery system in siRNA effect, 
      followed by FuGENE6. There was no significant difference between Lipofectamine 
      and FuGENE6 as a delivery system of antisense ODNs.
FAU - Ebadi, Padideh
AU  - Ebadi P
AD  - Department of Biology, Science and Research Branch, Islamic Azad University.
FAU - Karimi, Mohammad Hossein
AU  - Karimi MH
FAU - Pourfathollah, Ali Akbar
AU  - Pourfathollah AA
FAU - Saheb Ghadam Lotfi, Abbas
AU  - Saheb Ghadam Lotfi A
FAU - Soheili, Zahra Soheila
AU  - Soheili ZS
FAU - Samiee, Shahram
AU  - Samiee S
FAU - Hajati, Smerdis
AU  - Hajati S
FAU - Nadali, Fatemeh
AU  - Nadali F
FAU - Geramizadeh, Bita
AU  - Geramizadeh B
FAU - Moazzeni, Seyyed Mohammad
AU  - Moazzeni SM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran J Immunol
JT  - Iranian journal of immunology : IJI
JID - 101282932
RN  - 0 (CD40 Antigens)
RN  - 0 (FuGene)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Lipids)
RN  - 0 (Lipofectamine)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/metabolism
MH  - CD40 Antigens/*genetics/metabolism
MH  - Cell Line
MH  - Cell Survival
MH  - Dendritic Cells/metabolism
MH  - Down-Regulation/*genetics
MH  - Indicators and Reagents/chemistry
MH  - Lipids/*chemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligodeoxyribonucleotides, Antisense/chemistry/*genetics/metabolism
MH  - RNA, Small Interfering/chemistry/*genetics/metabolism
MH  - Spleen/cytology
MH  - Transfection/*methods
EDAT- 2009/03/19 09:00
MHDA- 2009/07/17 09:00
CRDT- 2009/03/19 09:00
PHST- 2009/03/19 09:00 [entrez]
PHST- 2009/03/19 09:00 [pubmed]
PHST- 2009/07/17 09:00 [medline]
AID - 01 [pii]
PST - ppublish
SO  - Iran J Immunol. 2009 Mar;6(1):1-11.