PMID- 19295142
OWN - NLM
STAT- MEDLINE
DCOM- 20090413
LR  - 20211020
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 29
IP  - 11
DP  - 2009 Mar 18
TI  - Epithelial-derived brain-derived neurotrophic factor is required for gustatory 
      neuron targeting during a critical developmental period.
PG  - 3354-64
LID - 10.1523/JNEUROSCI.3970-08.2009 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is expressed in epithelial targets of 
      gustatory neurons (i.e., fungiform papillae) before their innervation, and BDNF 
      overexpression in nontaste regions of the tongue misdirects gustatory axons to 
      these sites, suggesting that BDNF is necessary for gustatory axons to locate and 
      innervate their correct targets during development. To test this hypothesis, we 
      examined the targeting of taste neurons in BDNF-null mice (bdnf(-/-)). Analysis 
      of bdnf(-/-) mice using a combination of DiI labeling and electron microscopy 
      revealed that taste regions were not innervated by gustatory axons. Instead, 
      branching was increased and many nontaste regions were innervated. The increased 
      branching by gustatory axons in these animals was facilitated by neurotrophin 4 
      (NT4), because branching was virtually eliminated in bdnf(-/-)/nt4(-/-) mice. No 
      abnormalities in gustatory innervation patterns and targeting were observed in 
      nt4(-/-) mice. Conditional removal of BDNF selectively in epithelial cells 
      disrupted targeting at the tongue tip, where gene recombination removed bdnf by 
      embryonic day 13.5 (E13.5). However, innervation patterns were normal in the 
      midregion and caudal portions of the tongue, where gene recombination did not 
      occur until E14.5. These findings demonstrate that BDNF derived from gustatory 
      epithelia is required for gustatory axons to correctly locate and innervate 
      fungiform papillae. In addition, they show that BDNF-mediated targeting is 
      restricted to a critical period of development, on or before E13.5.
FAU - Ma, Liqun
AU  - Ma L
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, Kentucky 40292, USA.
FAU - Lopez, Grace F
AU  - Lopez GF
FAU - Krimm, Robin F
AU  - Krimm RF
LA  - eng
GR  - R01 DC007176-02/DC/NIDCD NIH HHS/United States
GR  - R01 DC007176-04/DC/NIDCD NIH HHS/United States
GR  - R01 DC007176-01/DC/NIDCD NIH HHS/United States
GR  - R01 DC007176-05/DC/NIDCD NIH HHS/United States
GR  - R01 DC007176-03/DC/NIDCD NIH HHS/United States
GR  - DC007176/DC/NIDCD NIH HHS/United States
GR  - R01 DC007176/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Epithelial Cells/cytology/*physiology
MH  - Female
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Gene Targeting/methods
MH  - Mice
MH  - Mice, Knockout
MH  - Neurons/cytology/*physiology
MH  - Pregnancy
MH  - Taste/physiology
MH  - Taste Buds/cytology/*embryology/*physiology
MH  - Tongue/cytology/embryology/physiology
PMC - PMC2683425
MID - NIHMS103410
EDAT- 2009/03/20 09:00
MHDA- 2009/04/14 09:00
PMCR- 2009/09/18
CRDT- 2009/03/20 09:00
PHST- 2009/03/20 09:00 [entrez]
PHST- 2009/03/20 09:00 [pubmed]
PHST- 2009/04/14 09:00 [medline]
PHST- 2009/09/18 00:00 [pmc-release]
AID - 29/11/3354 [pii]
AID - 3462637 [pii]
AID - 10.1523/JNEUROSCI.3970-08.2009 [doi]
PST - ppublish
SO  - J Neurosci. 2009 Mar 18;29(11):3354-64. doi: 10.1523/JNEUROSCI.3970-08.2009.