PMID- 19296259
OWN - NLM
STAT- MEDLINE
DCOM- 20100216
LR  - 20090319
IS  - 1671-0274 (Print)
IS  - 1671-0274 (Linking)
VI  - 12
IP  - 2
DP  - 2009 Mar
TI  - [Clinicopathological significance of expression and amplification of 
      P21-activated kinase 1 gene in colorectal carcinoma].
PG  - 185-8
AB  - OBJECTIVE: To investigate the clinicopathological value of the expression and 
      amplification of P21-activated kinase 1 gene (PAK1) in colorectal carcinoma(CRC). 
      METHODS: Immunohistochemistry (IHC), fluorescence in situ hybridization (FISH) 
      and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) 
      methods were used to examine the protein expression, amplification of PAK1 and 
      cell apoptosis in 80 cases of CRC and 30 cases of colorectal adenoma by tissue 
      microarray. RESULTS: IHC showed an overexpression of PAK1 protein in 26% of 
      colorectal adenomas and 62% of CRCs. Significant association was found between 
      expression of PAK1 and tumor histological grade as well as tumor clinical 
      stage(P<0.05). In poor-differentiated(G(3)) CRCs, PAK1 expression in 90% 
      carcinoma was up-regulated, which was significantly higher than that in tumors of 
      G(1/2)(51%). Overexpression of PAK1 was detected in 78% of CRCs in later clinical 
      stages (Dukes C, D), which was significantly higher than that in early clinical 
      stages (Dukes A,B, 53%). In addition, negative correlation between PAK1 
      overexpression and cell apoptosis was observed in these CRC cohorts(P<0.05). FISH 
      revealed that amplification of PAK1 gene was examined in only 3% CRCs. 
      CONCLUSIONS: Overexpression of PAK1 protein may play an important role in 
      development and progression of colorectal neoplasms and it is closely associated 
      with the malignant histological and invasive phenotype of CRCs. The expression of 
      PAK1 in CRC may be used as one of the new molecular markers in predicting tumors 
      malignant potential and progression.
FAU - Li, Zhi-xun
AU  - Li ZX
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou 510080, China.
FAU - Zeng, Sui-de
AU  - Zeng SD
FAU - Liu, Yong-dong
AU  - Liu YD
FAU - Liao, Yi-ji
AU  - Liao YJ
FAU - Hua, Wen-feng
AU  - Hua WF
FAU - Lin, Feng
AU  - Lin F
FAU - Xie, Dan
AU  - Xie D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wei Chang Wai Ke Za Zhi
JT  - Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
JID - 101177990
RN  - EC 2.7.11.1 (PAK1 protein, human)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
SB  - IM
MH  - Apoptosis
MH  - Colorectal Neoplasms/*genetics/*pathology
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - p21-Activated Kinases/*genetics
EDAT- 2009/03/20 09:00
MHDA- 2010/02/17 06:00
CRDT- 2009/03/20 09:00
PHST- 2009/03/20 09:00 [entrez]
PHST- 2009/03/20 09:00 [pubmed]
PHST- 2010/02/17 06:00 [medline]
AID - 100002452008 [pii]
PST - ppublish
SO  - Zhonghua Wei Chang Wai Ke Za Zhi. 2009 Mar;12(2):185-8.