PMID- 19296260
OWN - NLM
STAT- MEDLINE
DCOM- 20100216
LR  - 20090319
IS  - 1671-0274 (Print)
IS  - 1671-0274 (Linking)
VI  - 12
IP  - 2
DP  - 2009 Mar
TI  - [Tissue-engineering nerve repairs pelvic autonomic nerve defects in Beagle dogs].
PG  - 189-92
AB  - OBJECTIVE: To investigate the outcome of repairing pelvic autonomic nerve defects 
      with the tissue-engineered nerve, in order to provide a new method and 
      experimental evidence for solving sexual disturbance induced by pelvic autonomic 
      nerve injury after radical resection of rectal carcinoma. METHODS: Bone marrow 
      stromal cells (BMSCs) were purified with density gradient centrifugation. A 10 mm 
      defect of hypogastric nerve was created in 9 Beagle dogs and the 18 hypogastric 
      nerves were randomly divided into three groups. Group A: nerve defects bridged 
      with copolymer of lactic and glycolic acids (PLGA) tube containing BMSCs and 
      collagen protein sponge. Group B: with PLGA tube only containing collagen protein 
      sponge. Group C: with autologous nerve graft. The effect of nerve recovery was 
      evaluated by morphology, HE staining, neurofilament immunohistochemistry 
      staining, electron microscope scanning and measurement quantity of new axon 12 
      weeks after the transplantation. RESULTS: Twelve weeks after the transplantation, 
      degradation of PLGA tubes showed in group A and group B. The nerves regenerated 
      through defect area to distal end. The density of regeneration nerve fiber in 
      group A and group C were better than that in group B. The difference was 
      significant between group A or group C and group B (P<0.05), and no significant 
      difference was observed between group A and group C(P>0.05). CONCLUSION: 
      Tissue-engineered nerve, which is constructed by BMSCs mixed with collagen 
      protein sponge and PLGA tube, can be used to bridge and repair the pelvic 
      autonomic nerve defect.
FAU - Li, Ming-zhe
AU  - Li MZ
AD  - Department of Gastrointestinopancreatic Surgery, The First Affiliated Hospital, 
      Sun Yat -sen University, Guangzhou 510080, China.
FAU - Song, Xin-ming
AU  - Song XM
FAU - Zhang, Li
AU  - Zhang L
FAU - Chen, Zhi-hui
AU  - Chen ZH
FAU - He, Yu-long
AU  - He YL
FAU - Huang, Yi-hua
AU  - Huang YH
FAU - Cai, Shi-rong
AU  - Cai SR
FAU - Zhan, Wen-hua
AU  - Zhan WH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wei Chang Wai Ke Za Zhi
JT  - Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
JID - 101177990
SB  - IM
MH  - Animals
MH  - Artificial Organs
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dogs
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Motor Neuron Disease/*surgery
MH  - *Nerve Regeneration
MH  - Nerve Tissue
MH  - Pelvis/*innervation
MH  - *Tissue Engineering/methods
EDAT- 2009/03/20 09:00
MHDA- 2010/02/17 06:00
CRDT- 2009/03/20 09:00
PHST- 2009/03/20 09:00 [entrez]
PHST- 2009/03/20 09:00 [pubmed]
PHST- 2010/02/17 06:00 [medline]
AID - 100002572008 [pii]
PST - ppublish
SO  - Zhonghua Wei Chang Wai Ke Za Zhi. 2009 Mar;12(2):189-92.