PMID- 19297473
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 11
DP  - 2009 Jun
TI  - Differential effect of human herpesvirus 6A on cell division and apoptosis among 
      naive and central and effector memory CD4+ and CD8+ T-cell subsets.
PG  - 5442-50
LID - 10.1128/JVI.00106-09 [doi]
AB  - The immune responses of naive and different memory subsets of CD4(+) and CD8(+) T 
      cells to human herpesvirus 6 (HHV-6) have not been previously investigated. We 
      show that HHV-6A induces cell division, as measured by 5,6-carboxyfluorescein 
      succinimidyl ester dye and flow cytometry, predominantly in two populations of 
      effector memory CD4(+) and CD8(+) T cells (T(EM) and T(EMRA)); naive (T(N)) and 
      central memory (T(CM)) CD4(+) and CD8(+) T cells showed almost no cell division. 
      In contrast, HHV-6A induced apoptosis primarily in T(N) and T(CM) CD4(+) and 
      CD8(+) T cells, whereas T(EM) and T(EMRA) CD4(+) and CD8(+) T cells were 
      resistant to HHV-6A-induced apoptosis. HHV-6A-induced apoptosis was associated 
      with activation of caspase-8, caspase-9, and caspase-3, suggesting the 
      involvement of death receptor and mitochondrial signaling pathways. In addition, 
      HHV-6A induced secretion of interleukin-6 (IL-6), tumor necrosis factor alpha 
      (TNF-alpha), IL-8, and gamma interferon by peripheral blood mononuclear cells; 
      TNF-alpha secretion was observed exclusively from CCR7(+) (T(N) plus T(CM)) 
      CD4(+) T cells. These data show that HHV-6 differentially influences the 
      functions of naive T cells and different subsets of memory CD4(+) and CD8(+) T 
      cells, which in part may be due to differential susceptibility to HHV-6A-induced 
      apoptosis.
FAU - Gupta, Sudhir
AU  - Gupta S
AD  - Division of Basic and Clinical Immunology, University of California, Irvine, 
      California 92697, USA. sgupta@uci.edu
FAU - Agrawal, Sudhanshu
AU  - Agrawal S
FAU - Gollapudi, Sastry
AU  - Gollapudi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090318
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Cytokines)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Apoptosis/*immunology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - Cell Division/immunology
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis/immunology
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Immunity, Innate/*immunology
MH  - Immunologic Memory/*immunology
MH  - Membrane Cofactor Protein/immunology
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - T-Lymphocyte Subsets/cytology/*immunology
MH  - Up-Regulation/immunology
PMC - PMC2681935
EDAT- 2009/03/20 09:00
MHDA- 2009/05/30 09:00
PMCR- 2009/12/01
CRDT- 2009/03/20 09:00
PHST- 2009/03/20 09:00 [entrez]
PHST- 2009/03/20 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
PHST- 2009/12/01 00:00 [pmc-release]
AID - JVI.00106-09 [pii]
AID - 0106-09 [pii]
AID - 10.1128/JVI.00106-09 [doi]
PST - ppublish
SO  - J Virol. 2009 Jun;83(11):5442-50. doi: 10.1128/JVI.00106-09. Epub 2009 Mar 18.