PMID- 19301427
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20211020
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 87
IP  - 15
DP  - 2009 Nov 15
TI  - Distinct modes of migration position oligodendrocyte precursors for localized 
      cell division in the developing spinal cord.
PG  - 3320-30
LID - 10.1002/jnr.22058 [doi]
AB  - Establishment of the cytoarchitecture of the central nervous system reflects the 
      stereotyped cell migration and proliferation of precursor cells during 
      development. In vitro analyses have provided extensive information on the control 
      of proliferation and differentiation of oligodendrocyte precursors (OPCs), but 
      less is known about the migratory behavior of these cells in vivo. Here we 
      utilize a transgenic mouse line expressing enhanced green fluorescent protein 
      (EGFP) under the proteolipid protein promoter (PLP-EGFP mice) to visualize 
      directly the behaviors of OPCs in developing spinal cord slices. During early 
      development, OPCs disperse from their origin at the ventricular zone by using 
      saltatory migration. This involves orientation of the cell with a leading edge 
      toward the pial surface and alternating stationary and fast-moving phases and 
      dramatic shape changes. Once cells exit the ventricular zone, they exhibit an 
      exploratory mode of migration characterized by persistent translocation without 
      dramatic changes in cell morphology. The control of migration, proliferation, and 
      cytokinesis of OPCs appear to be closely linked. In netrin-1 mutant spinal cords 
      that lack dispersal cues, OPC migration rates were not significantly different, 
      but the trajectories were altered, and numbers of migrating cells were 
      dramatically reduced. In contrast to DNA replication that occurs at the 
      ventricular zone or throughout the spinal cord neuropil, cell division or 
      cytokinesis of OPCs occurs predominantly at the interface between gray and white 
      matters, with the majority of cleavage planes parallel to the pial surface. These 
      studies suggest that positional cues are critical for regulating OPC behavior 
      during spinal cord development.
FAU - Tsai, Hui-Hsin
AU  - Tsai HH
AD  - Center for Translational Neuroscience, Department of Neurosciences, Case Western 
      Reserve University School of Medicine, Cleveland, Ohio, USA.
FAU - Macklin, Wendy B
AU  - Macklin WB
FAU - Miller, Robert H
AU  - Miller RH
LA  - eng
GR  - R01 NS036674/NS/NINDS NIH HHS/United States
GR  - R01 NS025304/NS/NINDS NIH HHS/United States
GR  - NS36674/NS/NINDS NIH HHS/United States
GR  - R01 NS030800/NS/NINDS NIH HHS/United States
GR  - NS30800/NS/NINDS NIH HHS/United States
GR  - R01 NS030800-14/NS/NINDS NIH HHS/United States
GR  - R37 NS036674/NS/NINDS NIH HHS/United States
GR  - P30 CA043703/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Myelin Proteolipid Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Ntn1 protein, mouse)
RN  - 0 (Ntn1 protein, rat)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 158651-98-0 (Netrin-1)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cell Division/*physiology
MH  - Cell Movement/*physiology
MH  - Cell Polarity/genetics
MH  - Cell Proliferation
MH  - Cell Shape/genetics
MH  - Cells, Cultured
MH  - Cytokinesis/physiology
MH  - Green Fluorescent Proteins/chemistry/genetics/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Myelin Proteolipid Protein/chemistry/genetics/metabolism
MH  - Nerve Growth Factors/genetics
MH  - Netrin-1
MH  - Oligodendroglia/cytology/*physiology
MH  - Organ Culture Techniques
MH  - Rats
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Spinal Cord/cytology/*embryology/*physiology
MH  - Stem Cells/cytology/*physiology
MH  - Tumor Suppressor Proteins/genetics
PMC - PMC2861839
MID - NIHMS196656
EDAT- 2009/03/21 09:00
MHDA- 2010/02/02 06:00
PMCR- 2010/04/30
CRDT- 2009/03/21 09:00
PHST- 2009/03/21 09:00 [entrez]
PHST- 2009/03/21 09:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
PHST- 2010/04/30 00:00 [pmc-release]
AID - 10.1002/jnr.22058 [doi]
PST - ppublish
SO  - J Neurosci Res. 2009 Nov 15;87(15):3320-30. doi: 10.1002/jnr.22058.