PMID- 19302034
OWN - NLM
STAT- MEDLINE
DCOM- 20090821
LR  - 20161018
IS  - 1434-6621 (Print)
IS  - 1434-6621 (Linking)
VI  - 47
IP  - 5
DP  - 2009
TI  - MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer 
      diagnosis.
PG  - 530-6
LID - 10.1515/CCLM.2009.126 [doi]
AB  - BACKGROUND: Detection of tumor-associated genetic alterations in plasma of cancer 
      patients has recently been suggested to be an accurate method for detecting early 
      or recurrent cancer. METHODS: We performed quantitative real-time PCR for MYC and 
      GAPDH in tissue and plasma samples of 57 patients with gastric cancer and in 
      plasma of 79 cancer-free individuals. We also performed two-color MYC 
      fluorescence in situ hybridization (FISH) in tissue from the 57 patients with 
      gastric cancer. RESULTS: The tissue MYC/GAPDH ratio by real-time PCR was 
      significantly correlated with MYC status by FISH (p<0.001). The mean ratio of 
      plasma MYC/GAPDH was 5.226+/-3.578 (range: 1.25-18.35) in gastric cancer 
      patients, and 2.436+/-0.881 (range: 1.00-5.00) in the healthy volunteers 
      (p<0.001). We used receiver-operating characteristics (ROC) curve analysis to 
      select two optimal plasma MYC/GAPDH cut-offs of 2.725 and 5.225. The sensitivity 
      and specificity were 75.4% and 76.9% at 2.725, 38.6% and 100% at 5.225, 
      respectively. The plasma MYC/GAPDH ratio from cancer patients was significantly 
      correlated with the tissue MYC/GAPDH ratio (p=0.009), and tissue MYC status by 
      FISH (p=0.024). CONCLUSIONS: These findings suggest that the plasma MYC/GAPDH 
      ratio, as determined by real-time PCR, may be an alternative non-invasive 
      approach for detecting gastric cancer.
FAU - Park, Kyoung Un
AU  - Park KU
AD  - Department of Laboratory Medicine, Seoul National University Bundang Hospital, 
      Seongnam, Korea.
FAU - Lee, Hee Eun
AU  - Lee HE
FAU - Park, Do Joong
AU  - Park DJ
FAU - Jung, Eun Ji
AU  - Jung EJ
FAU - Song, Junghan
AU  - Song J
FAU - Kim, Hyung-Ho
AU  - Kim HH
FAU - Choe, Gheeyoung
AU  - Choe G
FAU - Kim, Woo Ho
AU  - Kim WH
FAU - Lee, Hye Seung
AU  - Lee HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Biomarkers, Tumor)
RN  - 9007-49-2 (DNA)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Biomarkers, Tumor/*blood
MH  - Cell Nucleolus/genetics
MH  - Centromere/genetics
MH  - DNA/*blood/genetics
MH  - Female
MH  - Gene Amplification
MH  - Genes, myc/*genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/blood/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stomach Neoplasms/blood/*diagnosis/genetics/pathology
EDAT- 2009/03/24 09:00
MHDA- 2009/08/22 09:00
CRDT- 2009/03/24 09:00
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2009/08/22 09:00 [medline]
AID - 10.1515/CCLM.2009.126 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2009;47(5):530-6. doi: 10.1515/CCLM.2009.126.