PMID- 19302099
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20221207
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 106
IP  - 3
DP  - 2009 Mar
TI  - Proteomic analysis of lactose-starved Lactobacillus casei during stationary 
      growth phase.
PG  - 764-73
LID - 10.1111/j.1365-2672.2008.03961.x [doi]
AB  - AIMS: Starvation stress is a condition that nonstarter lactic acid bacteria 
      (NSLAB) normally encounter. This study was aimed to investigate 
      starvation-induced proteins in Lactobacillus casei during stationary growth 
      phase. METHODS AND RESULTS: The impact of carbohydrate starvation on L. casei 
      GCRL163 was investigated using two different media (a modified de Man, Rogosa and 
      Sharpe broth and a semi-defined medium). Cells were grown in the presence of 
      excess lactose (1%) or starvation (0%) and differences in the patterns of 
      one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 
      two-dimensional electrophoresis of the cytosolic protein fractions were 
      investigated. Differentially regulated proteins were identified by MALDI-TOF/TOF 
      mass spectrometry. Many differentially regulated proteins were enzymes of various 
      metabolic pathways involved in carbohydrate metabolism to yield energy. 
      Differences in protein expression were also observed in the two culture 
      conditions tested in this experiment. CONCLUSION: Numerous glycolytic enzymes 
      were differentially regulated under lactose starvation. The differential 
      expression of these glycolytic enzymes suggests a potential survival strategy 
      under harsh growth conditions (i.e. lactose starvation). SIGNIFICANCE AND IMPACT 
      OF THE STUDY: This paper reports improved understanding of stress responses and 
      survival mechanism of NSLAB under lactose-depleted cheese-ripening condition. 
      This knowledge of how NSLAB bacteria adapt to lactose starvation could be applied 
      to predict the performances of bacteria in other industrial applications.
FAU - Hussain, M A
AU  - Hussain MA
AD  - School of Agriculture and Food Systems, The University of Melbourne, Australia. 
      ma.hussain@qut.edu.au
FAU - Knight, M I
AU  - Knight MI
FAU - Britz, M L
AU  - Britz ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Bacterial Proteins)
RN  - 0 (Culture Media)
RN  - J2B2A4N98G (Lactose)
SB  - IM
MH  - Bacterial Proteins/*isolation & purification/*metabolism
MH  - Culture Media
MH  - Cytosol/metabolism
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Lacticaseibacillus casei/growth & development/*metabolism
MH  - Lactose/metabolism
MH  - Proteomics/methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2009/03/24 09:00
MHDA- 2010/04/17 06:00
CRDT- 2009/03/24 09:00
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
AID - JAM3961 [pii]
AID - 10.1111/j.1365-2672.2008.03961.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2009 Mar;106(3):764-73. doi: 10.1111/j.1365-2672.2008.03961.x.