PMID- 19302273
OWN - NLM
STAT- MEDLINE
DCOM- 20091001
LR  - 20131121
IS  - 1399-3062 (Electronic)
IS  - 1398-2273 (Linking)
VI  - 11
IP  - 3
DP  - 2009 Jun
TI  - Mycophenolic acid inhibits PMA-induced activation of the neutrophil respiratory 
      burst.
PG  - 235-40
LID - 10.1111/j.1399-3062.2009.00382.x [doi]
AB  - Mycophenolate mofetil (MMF) is commonly used in immunosuppressive regimens for 
      solid organ transplantation. There is evidence that the hydrolyzed active agent 
      mycophenolic acid (MPA) causes the endothelial depletion of intracellular 
      guanosine 5'-triphosphate (GTP) levels. This depletion may cause inactivation of 
      nicotinamide adenine dinucleotide phosphate oxidase. The purpose of the present 
      study was to examine the impact of MPA on the neutrophil respiratory burst and 
      phagocytic activity using flow cytometry. In whole blood of healthy volunteers, 2 
      different doses of MPA (1 and 10 mumol/L) did not alter hydrogen peroxide 
      production of neutrophils induced by receptor-dependent activators. In contrast, 
      MPA inhibits the protein kinase C (PKC)-mediated hydrogen peroxide production by 
      phorbol 12-myristate 13-acetate (PMA) in a time-dependent manner (negative: 21.17 
      +/- 1.64 vs. 120 min: 14.46 +/- 1.28 mean fluorescence intensity, incubation with 
      1 mumol/L MPA). In conclusion, our results corroborated that the neutrophil 
      respiratory burst activity of healthy volunteers, induced by either 
      formyl-methionyl-leucylphenylalanine (fMLP), priming with tumor necrosis factor 
      alpha followed by fMLP or Escherichia coli and neutrophil phagocytic capacity, 
      were not significantly affected after MPA treatment. We also could demonstrate 
      that the hydrogen peroxide production of neutrophils decreased in response to the 
      PKC activator PMA in a time-dependent manner.
FAU - Juttner, B
AU  - Juttner B
AD  - Department of Anesthesiology, Hannover Medical School, Hannover, Germany. 
      juettner.bjoern@mh-hannover.de
FAU - Bencel, M
AU  - Bencel M
FAU - Weissig, A
AU  - Weissig A
FAU - Studzinski, A
AU  - Studzinski A
FAU - Stenger, K
AU  - Stenger K
FAU - Scheinichen, D
AU  - Scheinichen D
LA  - eng
PT  - Journal Article
DEP - 20090315
PL  - Denmark
TA  - Transpl Infect Dis
JT  - Transplant infectious disease : an official journal of the Transplantation 
      Society
JID - 100883688
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - HU9DX48N0T (Mycophenolic Acid)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Male
MH  - Mycophenolic Acid/*pharmacology
MH  - Neutrophils/*drug effects/immunology/metabolism
MH  - Phagocytosis/*drug effects
MH  - Respiratory Burst/*drug effects
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Young Adult
EDAT- 2009/03/24 09:00
MHDA- 2009/10/02 06:00
CRDT- 2009/03/24 09:00
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2009/10/02 06:00 [medline]
AID - TID382 [pii]
AID - 10.1111/j.1399-3062.2009.00382.x [doi]
PST - ppublish
SO  - Transpl Infect Dis. 2009 Jun;11(3):235-40. doi: 10.1111/j.1399-3062.2009.00382.x. 
      Epub 2009 Mar 15.