PMID- 19303025
OWN - NLM
STAT- MEDLINE
DCOM- 20090528
LR  - 20211203
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 84
IP  - 21-22
DP  - 2009 May 22
TI  - Caffeic acid phenethyl ester accumulates beta-catenin through GSK-3beta and 
      participates in proliferation through mTOR in C2C12 cells.
PG  - 755-9
LID - 10.1016/j.lfs.2009.03.004 [doi]
AB  - AIM: The aim of this study is to characterize the roles of caffeic acid phenethyl 
      ester (CAPE) in the skeletal muscle cells. MAIN METHODS: We performed 
      immunoblotting assay using various phosphorylation specific antibodies. KEY 
      FINDINGS: We found that CAPE induces rapid and transient phosphorylation of 
      glycogen synthase kinase (GSK)-3beta in a phosphoinositide 3-kinase 
      (PI3K)-dependent manner. CAPE also decreases phosphorylation of beta-catenin, 
      ultimately leading to beta-catenin accumulation. In addition, we demonstrated 
      that CAPE activated the mammalian target of rapamycin (mTOR)-p70 S6 ribosomal 
      kinase (S6K) and also stimulated extracellular signal-regulated kinase (ERK). The 
      inhibition of mTOR blocked CAPE-induced ERK phosphorylation. SIGNIFICANCE: Our 
      results suggest that CAPE may act through beta-catenin accumulation via 
      stimulation of GSK-3beta and may also participate in cellular proliferation 
      through the mTOR-ERK pathway.
FAU - Lee, Eun Soo
AU  - Lee ES
AD  - Department of Anatomy, Korea University College of Medicine, Seoul 136-705, 
      Republic of Korea.
FAU - Lee, Jung-Ok
AU  - Lee JO
FAU - Lee, Soo Kyung
AU  - Lee SK
FAU - Kim, Ji Hae
AU  - Kim JH
FAU - Jung, Jin Hee
AU  - Jung JH
FAU - Keum, Bora
AU  - Keum B
FAU - Park, Sun-Hwa
AU  - Park SH
FAU - Kim, Hyeon Soo
AU  - Kim HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090318
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Actins)
RN  - 0 (Caffeic Acids)
RN  - 0 (Indicators and Reagents)
RN  - 0 (beta Catenin)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Gsk3b protein, mouse)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
RN  - G960R9S5SK (caffeic acid phenethyl ester)
RN  - ML9LGA7468 (Phenylethyl Alcohol)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Caffeic Acids/*metabolism
MH  - Cell Line
MH  - Cell Proliferation
MH  - Glycogen Synthase Kinase 3/*physiology
MH  - Glycogen Synthase Kinase 3 beta
MH  - Indicators and Reagents
MH  - Mice
MH  - Myoblasts/metabolism
MH  - Phenylethyl Alcohol/*analogs & derivatives/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Protein Kinases/*biosynthesis/genetics
MH  - Proto-Oncogene Proteins c-akt/physiology
MH  - TOR Serine-Threonine Kinases
MH  - beta Catenin/*metabolism
EDAT- 2009/03/24 09:00
MHDA- 2009/05/29 09:00
CRDT- 2009/03/24 09:00
PHST- 2008/10/02 00:00 [received]
PHST- 2009/03/03 00:00 [revised]
PHST- 2009/03/06 00:00 [accepted]
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2009/05/29 09:00 [medline]
AID - S0024-3205(09)00118-0 [pii]
AID - 10.1016/j.lfs.2009.03.004 [doi]
PST - ppublish
SO  - Life Sci. 2009 May 22;84(21-22):755-9. doi: 10.1016/j.lfs.2009.03.004. Epub 2009 
      Mar 18.