PMID- 19303894
OWN - NLM
STAT- MEDLINE
DCOM- 20090609
LR  - 20161125
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 237
IP  - 2
DP  - 2009 Jun 1
TI  - Maternal exposure to anti-androgenic compounds, vinclozolin, flutamide and 
      procymidone, has no effects on spermatogenesis and DNA methylation in male rats 
      of subsequent generations.
PG  - 178-87
LID - 10.1016/j.taap.2009.03.004 [doi]
AB  - To verify whether anti-androgens cause transgenerational effects on 
      spermatogenesis and DNA methylation in rats, gravid Crl:CD(SD) female rats (4 or 
      5/group, gestational day (GD) 0=day sperm detected) were intraperitoneally 
      treated with anti-androgenic compounds, such as vinclozolin (100 mg/kg/day), 
      procymidone (100 mg/kg/day), or flutamide (10 mg/kg/day), from GD 8 to GD 15. 
      Testes were collected from F1 male pups at postnatal day (PND) 6 for DNA 
      methylation analysis of the region (210 bp including 7 CpG sites) within the 
      lysophospholipase gene by bisulfite DNA sequencing method. F0 and F1 males 
      underwent the sperm analysis (count, motility and morphology), followed by DNA 
      methylation analysis of the sperm. Remaining F1 males were cohabited with 
      untreated-females to obtain F2 male pups for subsequent DNA methylation analysis 
      of the testes at PND 6. These analyses showed no effects on spermatogenesis and 
      fertility in F1 males of any treatment group. DNA methylation status in testes 
      (F1 and F2 pups at PND 6) or sperms (F1 males at 13 weeks old) of the treatment 
      groups were comparable to the control at all observation points, although DNA 
      methylation rates in testes were slightly lower than those in sperm. In F0 males, 
      no abnormalities in the spermatogenesis, fertility and DNA methylation status of 
      sperm were observed. No transgenerational abnormalities of spermatogenesis and 
      DNA methylation status caused by anti-androgenic compounds were observed.
FAU - Inawaka, Kunifumi
AU  - Inawaka K
AD  - Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka, 
      Japan. inawaka@sc.sumitomo-chem.co.jp
FAU - Kawabe, Mayumi
AU  - Kawabe M
FAU - Takahashi, Satoru
AU  - Takahashi S
FAU - Doi, Yuko
AU  - Doi Y
FAU - Tomigahara, Yoshitaka
AU  - Tomigahara Y
FAU - Tarui, Hirokazu
AU  - Tarui H
FAU - Abe, Jun
AU  - Abe J
FAU - Kawamura, Satoshi
AU  - Kawamura S
FAU - Shirai, Tomoyuki
AU  - Shirai T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090319
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Androgen Antagonists)
RN  - 0 (Bridged Bicyclo Compounds)
RN  - 0 (Fungicides, Industrial)
RN  - 0 (Oxazoles)
RN  - 76W6J0943E (Flutamide)
RN  - EC 3.1.1.5 (Lysophospholipase)
RN  - EC2FI67U2Y (procymidone)
RN  - JJ258EZN1I (vinclozolin)
SB  - IM
MH  - Androgen Antagonists/toxicity
MH  - Animals
MH  - Base Sequence
MH  - Bridged Bicyclo Compounds/*toxicity
MH  - DNA Methylation/*drug effects
MH  - Female
MH  - Flutamide/*toxicity
MH  - Fungicides, Industrial/toxicity
MH  - Lysophospholipase/genetics
MH  - Male
MH  - Oxazoles/*toxicity
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*chemically induced
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spermatogenesis/*drug effects
MH  - Spermatozoa
MH  - Testis/drug effects/pathology
EDAT- 2009/03/24 09:00
MHDA- 2009/06/10 09:00
CRDT- 2009/03/24 09:00
PHST- 2008/12/24 00:00 [received]
PHST- 2009/03/10 00:00 [revised]
PHST- 2009/03/12 00:00 [accepted]
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2009/06/10 09:00 [medline]
AID - S0041-008X(09)00116-1 [pii]
AID - 10.1016/j.taap.2009.03.004 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2009 Jun 1;237(2):178-87. doi: 
      10.1016/j.taap.2009.03.004. Epub 2009 Mar 19.