PMID- 19303919
OWN - NLM
STAT- MEDLINE
DCOM- 20090921
LR  - 20211020
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 161
IP  - 1
DP  - 2009 Jun 16
TI  - Seizure activity involved in the up-regulation of BDNF mRNA expression by 
      activation of central mu opioid receptors.
PG  - 301-10
LID - 10.1016/j.neuroscience.2009.03.020 [doi]
AB  - Chemical-induced seizures up-regulated brain-derived neurotrophic factor (BDNF) 
      mRNA expression. Intracerebroventricular (i.c.v.) administration of endogenous 
      opioids preferentially activating mu opioid receptor (MOR) could also increase 
      BDNF mRNA expression. The aim of this study was to determine to what extent 
      i.c.v. administration of synthetic MOR-selective agonists in rats can modulate 
      both seizure activity and up-regulation of BDNF mRNA expression. Effects and 
      potencies of i.c.v. administration of morphine and [D-Ala(2), N-Me-Phe(4), 
      Gly(5)-ol]-enkephalin (DAMGO), were directly investigated by scoring behavioral 
      seizures and measuring BDNF mRNA expression. In addition, effects of the opioid 
      receptor antagonist naloxone and antiepileptic drugs, diazepam, phenobarbital, 
      and valproate, on i.c.v. MOR agonist-induced behavioral seizures and 
      up-regulation of BDNF mRNA expression were determined. A single i.c.v. 
      administration of morphine (10-100 microg) or DAMGO (0.15-1.5 microg) 
      dose-dependently elicited behavioral seizures and increased BDNF mRNA expression 
      in the widespread brain regions. However, s.c. administration of MOR agonists 
      neither produced behavioral seizures nor increased BDNF mRNA expression. 
      Pretreatment with naloxone 1 mg/kg significantly reduced behavioral seizure 
      scores and the up-regulation of BDNF mRNA expression elicited by i.c.v. morphine 
      or DAMGO. Similarly, diazepam 10 mg/kg and phenobarbital 40 mg/kg significantly 
      blocked i.c.v. MOR agonist-induced actions. Pretreatment with valproate 300 mg/kg 
      only attenuated behavioral seizures, but it did not affect morphine-induced 
      increase of BDNF mRNA expression. This study provides supporting evidence that 
      seizure activity plays an important role in the up-regulation of BDNF mRNA 
      expression elicited by central MOR activation and that decreased inhibitory 
      action of GABAergic system through the modulation on GABA receptor synaptic 
      function by central MOR activation is involved in its regulation of BDNF mRNA 
      expression.
FAU - Zhang, H N
AU  - Zhang HN
AD  - Department of Biomedical Engineering, College of Engineering, 1101 Beal Avenue, 
      University of Michigan, Ann Arbor, MI 48109, USA.
FAU - Ko, M C
AU  - Ko MC
LA  - eng
GR  - R01 DA023213/DA/NIDA NIH HHS/United States
GR  - MH42251/MH/NIMH NIH HHS/United States
GR  - P01 MH042251/MH/NIMH NIH HHS/United States
GR  - DA13685/DA/NIDA NIH HHS/United States
GR  - R01 DA013685/DA/NIDA NIH HHS/United States
GR  - DA07281/DA/NIDA NIH HHS/United States
GR  - P50 DA000254/DA/NIDA NIH HHS/United States
GR  - R01 DA023213-02/DA/NIDA NIH HHS/United States
GR  - T32 DA007281/DA/NIDA NIH HHS/United States
GR  - R01 DA013685-01A1/DA/NIDA NIH HHS/United States
GR  - P50 DA000254-300015/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090319
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Anticonvulsants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Opioid, mu)
RN  - 100929-53-1 (Enkephalin, Ala(2)-MePhe(4)-Gly(5)-)
RN  - 36B82AMQ7N (Naloxone)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Animals
MH  - Anticonvulsants/pharmacology
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Drug Synergism
MH  - Enkephalin, Ala(2)-MePhe(4)-Gly(5)-/administration & dosage/pharmacology
MH  - Injections, Intraventricular
MH  - Male
MH  - Morphine/administration & dosage/pharmacology
MH  - Naloxone/pharmacology
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Opioid, mu/*agonists/antagonists & inhibitors
MH  - Seizures/*metabolism/physiopathology
MH  - Up-Regulation
PMC - PMC2680449
MID - NIHMS103727
EDAT- 2009/03/24 09:00
MHDA- 2009/09/22 06:00
PMCR- 2010/06/16
CRDT- 2009/03/24 09:00
PHST- 2008/10/29 00:00 [received]
PHST- 2009/03/07 00:00 [revised]
PHST- 2009/03/10 00:00 [accepted]
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2009/09/22 06:00 [medline]
PHST- 2010/06/16 00:00 [pmc-release]
AID - S0306-4522(09)00373-X [pii]
AID - 10.1016/j.neuroscience.2009.03.020 [doi]
PST - ppublish
SO  - Neuroscience. 2009 Jun 16;161(1):301-10. doi: 10.1016/j.neuroscience.2009.03.020. 
      Epub 2009 Mar 19.