PMID- 19306265
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20091027
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 24
IP  - 5
DP  - 2009 Sep-Oct
TI  - What happens with ageing: decline or remodeling of opsonin-independent phagocyte 
      oxidative activity?
PG  - 340-7
LID - 10.1002/bio.1115 [doi]
AB  - Changes that occur with age in the opsonin-independent oxidative activity of 
      peripheral phagocytes in whole blood were examined by means of luminol 
      chemiluminescence (LCL). The chemiluminescence was registered simultaneously by 
      non-stimulated and stimulated cells and the age-related alterations of total and 
      extracellular generation of reactive oxygen species (ROS) were studied using 
      model systems. It was found that the rate of phagocyte activation by the glass 
      surface of the measuring chambers, assessed by the time of the peak appearance 
      after the start of LCL response, increased. However, the maximum oxidative 
      activity and the integral oxidative capacity of the cells during adhesion, 
      evaluated by the maximum LCL intensity and the area under the LCL curve, 
      respectively, declined. No age-dependence of 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated oxidative cellular 
      activity for total ROS generation was detected. The maximum oxidative activity 
      and the integral oxidative capacity of peripheral phagocytes to generate 
      extracellular superoxide in response to fMLP was decreased. The likely causes for 
      the observed alterations in phagocyte function are discussed and an analysis of 
      the obtained results is given on the background of the contradictory data 
      published on phagocyte oxidative activity age-related changes.
FAU - Alexandrova, Margarita L
AU  - Alexandrova ML
AD  - Department of Biophysics, Medical University, Pleven, Bulgaria. 
      margalexandrova@hotmail.com
LA  - eng
PT  - Journal Article
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Opsonin Proteins)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aging/*metabolism
MH  - Female
MH  - Humans
MH  - Kinetics
MH  - Male
MH  - Middle Aged
MH  - Opsonin Proteins/*physiology
MH  - Oxidation-Reduction
MH  - Phagocytes/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Reference Values
MH  - Sex Factors
EDAT- 2009/03/24 09:00
MHDA- 2010/02/06 06:00
CRDT- 2009/03/24 09:00
PHST- 2009/03/24 09:00 [entrez]
PHST- 2009/03/24 09:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
AID - 10.1002/bio.1115 [doi]
PST - ppublish
SO  - Luminescence. 2009 Sep-Oct;24(5):340-7. doi: 10.1002/bio.1115.