PMID- 19307781
OWN - NLM
STAT- MEDLINE
DCOM- 20090522
LR  - 20090324
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 87
IP  - 4
DP  - 2009 Feb 27
TI  - Increased frequency and responsiveness of PSA-specific T cells after allogeneic 
      hematopoetic stem-cell transplantation.
PG  - 467-72
LID - 10.1097/TP.0b013e3181949c16 [doi]
AB  - BACKGROUND: Therapies for localized prostate cancer include curative surgery and 
      radiotherapy while treatment of metastatic disease is often inefficient. 
      Graft-versus-tumor effects of allogeneic stem-cell transplantation (ASCT) have 
      been described for several types of solid tumors but have not been reported for 
      prostate cancer. We, therefore, investigated the potential of ASCT as treatment 
      for noncurable prostate cancer. METHODS: A patient underwent ASCT from his human 
      leukocyte antigen (HLA)-identical sister as treatment for his metastatic prostate 
      adenocarcinoma. Frequencies of prostate-specific T cells in the peripheral blood 
      of the patient, ASCT donor and a group of control individuals were determined by 
      flow cytometry using pentameric HLA-A2 complexes containing peptides derived from 
      the prostate-specific antigen (PSA). Cytotoxic activity of PSA-peptide-specific T 
      cells against peptide-pulsed target cells was analyzed ex vivo by Cr-release 
      assays. RESULTS: Stable clinical and laboratory remission lasting more than 4 
      years was observed after ASCT. Using HLA-containing pentamers with PSA-derived 
      peptides we could detect prostate-specific CD8+ T cells in this patient at high 
      frequencies over several months. Furthermore, higher frequencies of PSA-specific 
      T cells were revealed in the blood of the patient and female controls when 
      compared with healthy males. CONCLUSIONS: Lymphocytes from the peripheral blood 
      of the recipient, but not from donor or other tested control individuals, 
      exhibited ex vivo cytotoxic activity against target cells pulsed with the 
      relevant synthetic peptides and efficiently expanded in vitro following specific 
      restimulations. Thus, the results of this study indicate that female to male ASCT 
      can increase the frequency and enhance specific-responsiveness of PSA-specific T 
      cells in transplant recipients.
FAU - Uhlin, Michael
AU  - Uhlin M
AD  - Centre for Allogeneic Stem Cell Transplantation and Division of Clinical 
      Immunology, Karolinska University Hospital Huddinge, Stockholm, Sweden. 
      michael.uhlin@ki.se
FAU - Okas, Mantas
AU  - Okas M
FAU - Karlsson, Helen
AU  - Karlsson H
FAU - Gertow, Jens
AU  - Gertow J
FAU - Henningsohn, Lars
AU  - Henningsohn L
FAU - Ringden, Olle
AU  - Ringden O
FAU - Karre, Klas
AU  - Karre K
FAU - Levitsky, Victor
AU  - Levitsky V
FAU - Mattsson, Jonas
AU  - Mattsson J
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antigens, CD)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A2 Antigen)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Adenocarcinoma/immunology/pathology
MH  - Algorithms
MH  - Amino Acid Sequence
MH  - Antigens, CD/blood
MH  - Cell Survival
MH  - Epitopes/immunology
MH  - HLA-A2 Antigen/genetics
MH  - Hematopoietic Stem Cell Transplantation/*statistics & numerical data
MH  - Humans
MH  - Lymphocyte Transfusion
MH  - Male
MH  - Prostate-Specific Antigen/*immunology
MH  - Prostatic Neoplasms/immunology/pathology
MH  - Sex Characteristics
MH  - T-Lymphocytes/*immunology
MH  - Transplantation, Homologous
EDAT- 2009/03/25 09:00
MHDA- 2009/05/23 09:00
CRDT- 2009/03/25 09:00
PHST- 2009/03/25 09:00 [entrez]
PHST- 2009/03/25 09:00 [pubmed]
PHST- 2009/05/23 09:00 [medline]
AID - 00007890-200902270-00002 [pii]
AID - 10.1097/TP.0b013e3181949c16 [doi]
PST - ppublish
SO  - Transplantation. 2009 Feb 27;87(4):467-72. doi: 10.1097/TP.0b013e3181949c16.