PMID- 19307926
OWN - NLM
STAT- MEDLINE
DCOM- 20090518
LR  - 20191210
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Linking)
VI  - 38
IP  - 3
DP  - 2009 Apr
TI  - Neurogenin 3 and neurogenic differentiation 1 are retained in the cytoplasm of 
      multiple endocrine neoplasia type 1 islet and pancreatic endocrine tumor cells.
PG  - 259-66
LID - 10.1097/MPA.0b013e3181930818 [doi]
AB  - OBJECTIVES: To investigate if transcription factors involved in pancreatic 
      differentiation and regeneration are present in pancreatic endocrine tumors and 
      if they are differentially expressed in normal pancreas compared with multiple 
      endocrine neoplasia type 1 (MEN1) nontumorous pancreas. METHODS: The expression 
      of neurogenin 3 (NEUROG3), neurogenic differentiation 1 (NEUROD1), POU class 3 
      homeobox 4 (POU3F4), pancreatic duodenal homeobox factor 1 (PDX1), ribosomal 
      protein L10 (RPL10), delta-like 1 homolog (Drosophila; DLK1), and menin was 
      analyzed by immunohistochemistry in normal pancreas and pancreatic endocrine 
      tumors from 6 patients with MEN1 and 16 patients with sporadic tumors, as well as 
      pancreatic specimens from Men1 heterozygous and wild type mice. Quantitative 
      polymerase chain reaction was performed in a subset of human tumors. RESULTS: 
      Tumors and MEN1 nontumorous endocrine cells showed a prominent cytoplasmatic 
      NEUROG3 and NEUROD1 expression. These factors were significantly more expressed 
      in the cytoplasm of Men1 heterozygous mouse islet cells compared with wild type 
      islets; the latter showed an exclusively nuclear reactivity. The degree of 
      Pou3f4, Rpl10, and Dlk1 immunoreactivities differed significantly between islets 
      of heterozygous and wild type mice. The expressions of RPL10 and NEUROD1 were 
      prominent in the MEN1 human and heterozygous mouse exocrine pancreas. Insulinomas 
      had significantly higher PDX1 and DLK1 messenger RNA levels compared with other 
      tumor types. CONCLUSIONS: Transcription factors involved in pancreatic 
      development show altered expression and subcellular localization in MEN1 
      nontumorous pancreas and pancreatic endocrine tumors.
FAU - Lejonklou, Margareta H
AU  - Lejonklou MH
AD  - Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
FAU - Edfeldt, Katarina
AU  - Edfeldt K
FAU - Johansson, Terese A
AU  - Johansson TA
FAU - Stalberg, Peter
AU  - Stalberg P
FAU - Skogseid, Britt
AU  - Skogseid B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DLK1 protein, human)
RN  - 0 (Dlk1 protein, mouse)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NEUROD1 protein, human)
RN  - 0 (NEUROG3 protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurod1 protein, mouse)
RN  - 0 (Neurog3 protein, mouse)
RN  - 0 (POU Domain Factors)
RN  - 0 (POU3F4 protein, human)
RN  - 0 (RPL10 protein, human)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (Rpl10 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (pancreatic and duodenal homeobox 1 protein)
RN  - 147258-09-1 (Pou3f4 protein, mouse)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*metabolism
MH  - Calcium-Binding Proteins
MH  - Cytoplasm/metabolism
MH  - Female
MH  - Heterozygote
MH  - Homeodomain Proteins/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Insulinoma/genetics/*metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Multiple Endocrine Neoplasia Type 1/genetics/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - POU Domain Factors/metabolism
MH  - Pancreas, Exocrine/metabolism
MH  - Pancreatic Neoplasms/genetics/*metabolism
MH  - Ribosomal Protein L10
MH  - Ribosomal Proteins/metabolism
MH  - Trans-Activators/metabolism
EDAT- 2009/03/25 09:00
MHDA- 2009/05/19 09:00
CRDT- 2009/03/25 09:00
PHST- 2009/03/25 09:00 [entrez]
PHST- 2009/03/25 09:00 [pubmed]
PHST- 2009/05/19 09:00 [medline]
AID - 00006676-200904000-00004 [pii]
AID - 10.1097/MPA.0b013e3181930818 [doi]
PST - ppublish
SO  - Pancreas. 2009 Apr;38(3):259-66. doi: 10.1097/MPA.0b013e3181930818.