PMID- 19308950
OWN - NLM
STAT- MEDLINE
DCOM- 20091209
LR  - 20090907
IS  - 1098-2752 (Electronic)
IS  - 0738-1085 (Linking)
VI  - 29
IP  - 6
DP  - 2009
TI  - Effect of low-intensity pulsed ultrasound on the expression of neurotrophin-3 and 
      brain-derived neurotrophic factor in cultured Schwann cells.
PG  - 479-85
LID - 10.1002/micr.20644 [doi]
AB  - It is generally known that low-intensity pulsed ultrasound (LIPUS) accelerates 
      peripheral nerve tissue regeneration. However, the precise cellular mechanism 
      involved is still unclear. The purpose of this study was to determine how the 
      Schwann cells respond directly to LIPUS stimuli. Thus, we investigated the effect 
      of LIPUS on cell proliferation, neurotrophin-3 (NT-3), and brain-derived 
      neurotrophic factor (BDNF) mRNA expression in rat Schwann cells. Schwann cells 
      were enzymatically isolated from postnatal 1-3 day rat sciatic nerve tissue and 
      cultured in the six-well plate. The ultrasound was applied at a frequency of 1 
      MHz and an intensity of 100 mW/cm(2) spatial average temporal average for 5 
      minutes/day. The control group was cultured in the same way but without the 
      administration of ultrasound. Immunohistochemistry demonstrated that more than 
      98% of the experimental and control cells were positive for S-100, NT-3, and 
      BDNF. With 5-bromo-2'-deoxyuridine (BrdU) assay, the stimulated cells also 
      exhibited an increase in the rate of cell proliferation on days 4, 7, 10, and 14. 
      Further investigation found that mRNA expression of NT-3 was significantly 
      upregulated in experimental groups compared with the control 14 days after the 
      LIPUS stimulation (the ratio of NT-3/beta-actin was 0.56 +/- 0.13 vs. 0.41 +/- 
      0.09, P < 0.01), whereas the mRNA expression of BDNF was significantly 
      downregulated in experimental groups compared with the control (the ratio of 
      BDNF/beta-actin was 0.51 +/- 0.05 vs. 0.60 +/- 0.08, P < 0.05). These results 
      demonstrated that the application of LIPUS promotes cell proliferation and NT-3 
      gene expression in Schwann cells, and involved in the alteration of BDNF gene 
      expression.
CI  - Copyright 2009 Wiley-Liss, Inc. Microsurgery 2009.
FAU - Zhang, Hua
AU  - Zhang H
AD  - Department of Orthopaedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, China.
FAU - Lin, Xin
AU  - Lin X
FAU - Wan, Hong
AU  - Wan H
FAU - Li, Jun-Hua
AU  - Li JH
FAU - Li, Jia-Mou
AU  - Li JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microsurgery
JT  - Microsurgery
JID - 8309230
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Nerve Regeneration/*physiology
MH  - Neurotrophin 3/genetics/*metabolism
MH  - Probability
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Values
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Schwann Cells/*metabolism
MH  - Sciatic Nerve/cytology/metabolism/pathology
MH  - *Ultrasonic Therapy
EDAT- 2009/03/25 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/03/25 09:00
PHST- 2009/03/25 09:00 [entrez]
PHST- 2009/03/25 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1002/micr.20644 [doi]
PST - ppublish
SO  - Microsurgery. 2009;29(6):479-85. doi: 10.1002/micr.20644.