PMID- 19317061
OWN - NLM
STAT- MEDLINE
DCOM- 20110128
LR  - 20090325
IS  - 1000-503X (Print)
IS  - 1000-503X (Linking)
VI  - 31
IP  - 1
DP  - 2009 Feb
TI  - [Effects of very low-density lipoprotein on cellular lipid accumulation and the 
      expression of monocyte chemoattractant protein-1 in human mesangial cells].
PG  - 55-9
AB  - OBJECTIVE: To investigate the effects of very low-density lipoprotein (VLDL) on 
      cellular lipid accumulation and the expression of monocyte chemoattractant 
      protein-1 (MCP-1) in human mesangial cells. METHODS: An established stable human 
      mesangial cell line (HMCL) was used in all experiments. VLDL-induced cellular 
      lipid deposition was visualized by Oil Red O staining and analyzed quantitatively 
      by standard enzymatic procedures. MCP-1 mRNA and protein expression levels in 
      treated HMCLs were determined by real-time quantitative RT-PCR and enzyme-linked 
      immunosorbent assay, respectively. For adhesion study, HMCLs were treated with 
      VLDL for 12 hours, followed by a one-hour incubation with THP-1 cells. RESULTS: 
      VLDL induced cellular lipid accumulation in HMCLs in a time- (0-24 h) and dose- 
      (0-200 microg/ml) dependent manner, and the principal component of accumulated 
      lipid is triglyceride. In HMCLs, MCP-1 mRNA expression was promoted by VLDL in a 
      time- (0-6 h) and dose- (0-100 microg/ml) dependent manner, and VLDL also 
      enhanced MCP-1 secretion in a dose-dependent manner. Such an effect was 
      accompanied by increased adhesion of monocytes to HMCLs. CONCLUSIONS: VLDL can 
      induce cellular triglyceride accumulation and upregulate the expression of MCP-1 
      in human mesangial cells. Hence, VLDL may be involved in the pathogenesis of 
      lipid-mediated renal injury.
FAU - Li, Jing
AU  - Li J
AD  - Department of Nephrology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
FAU - Li, Hang
AU  - Li H
FAU - Wen, Yu-Bing
AU  - Wen YB
FAU - Li, Xue-Wang
AU  - Li XW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao
JT  - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
JID - 8006230
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Humans
MH  - Lipoproteins, VLDL/pharmacology/*toxicity
MH  - Mesangial Cells/cytology/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Triglycerides/*metabolism
EDAT- 2009/03/26 09:00
MHDA- 2011/02/01 06:00
CRDT- 2009/03/26 09:00
PHST- 2009/03/26 09:00 [entrez]
PHST- 2009/03/26 09:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
PST - ppublish
SO  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2009 Feb;31(1):55-9.