PMID- 19317740
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20111117
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 73
IP  - 4
DP  - 2009 Apr
TI  - HLA-B51 and haplotypic diversity of B-Cw associations: implications for matching 
      in unrelated hematopoietic stem cell transplantation.
PG  - 316-25
LID - 10.1111/j.1399-0039.2009.01215.x [doi]
AB  - In unrelated hematopoietic stem cell transplantation (HSCT), human leukocyte 
      antigen (HLA)-C locus incompatibilities occur frequently and are associated with 
      increased risk of posttransplant complications. Because HLA-B51 is associated 
      with a high rate of Cw disparities, we performed a comprehensive four-digit 
      typing analysis of 140 ABCDRB1 B51 genotypes proven by pedigree analysis and 311 
      unrelated donors selected for 75 B51-positive patients. In addition, 145 
      A1/Ax-B8/B51-DR3/DRx donors were HLA typed at a high-resolution level and tested 
      for three microsatellite (Msat) polymorphisms located in the HLA class I and III 
      regions. Based on these data sets, 182 different ABCDR haplotypes with 14 
      different B-Cw associations were detected. Rates of Cw mismatches were shown to 
      be highly correlated with the ABDRB1 haplotypes. We have computed 21 B51 
      haplotypes that disclose a high probability of HLA-C allele matching and 30 
      haplotypes with a low (<25%) probability. The HLA-C allele frequency profiles 
      were quite different in these two groups, with a more heterogeneous distribution 
      in the low matching probability group. HLA-Cw*1502 was inversely correlated with 
      the likelihood to identify a Cw-mismatched donor: it was present in 61% of the 
      high vs 18% of the low probability group (P < 0.0001). The analysis of three 
      Msats in the class I and III regions showed a higher allelic diversity in 
      B51-positive haplotypes compared with the conserved A1-B8-DR3 haplotype. HLA-B51 
      haplotypes therefore exhibit a high diversity at the level of B-Cw associations 
      and of non-HLA polymorphisms in the class I and III regions. Such heterogeneity 
      negatively impacts on overall matching in HSCT.
FAU - Bettens, F
AU  - Bettens F
AD  - Transplantation Immunology Unit, National Reference Laboratory for 
      Histocompatibility, University Hospital, 24 rue Micheli-du-Crest, Geneva, 
      Switzerland.
FAU - Nicoloso de Faveri, G
AU  - Nicoloso de Faveri G
FAU - Tiercy, J-M
AU  - Tiercy JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B51 Antigen)
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - *Genetic Variation
MH  - Genetics, Population
MH  - Genotype
MH  - HLA-B Antigens/*genetics
MH  - HLA-B51 Antigen
MH  - HLA-C Antigens/genetics
MH  - Haplotypes/*genetics
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Testing
MH  - Humans
MH  - Microsatellite Repeats
MH  - Registries
MH  - Tissue Donors
EDAT- 2009/03/26 09:00
MHDA- 2009/05/30 09:00
CRDT- 2009/03/26 09:00
PHST- 2009/03/26 09:00 [entrez]
PHST- 2009/03/26 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
AID - TAN1215 [pii]
AID - 10.1111/j.1399-0039.2009.01215.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2009 Apr;73(4):316-25. doi: 10.1111/j.1399-0039.2009.01215.x.