PMID- 19318089
OWN - NLM
STAT- MEDLINE
DCOM- 20090515
LR  - 20161125
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 382
IP  - 4
DP  - 2009 May 15
TI  - Upregulation of Beclin-1 expression and phosphorylation of Bcl-2 and p53 are 
      involved in the JNK-mediated autophagic cell death.
PG  - 726-9
LID - 10.1016/j.bbrc.2009.03.095 [doi]
AB  - Though the activation of c-Jun NH2-terminal kinase (JNK) has been reported to be 
      essential for autophagic cell death in response to various stressors, the 
      molecular links between JNK activation and autophagic cell death signaling remain 
      elusive. Here we report that, in the JNK-dependent autophagic cell death of 
      HCT116 cells induced by an agonistic single chain variable fragment antibody, 
      HW1, against human death receptor 5 (DR5), JNK activation upregulated Beclin-1 
      expression and induced Bcl-2 and p53 phosphorylation. Further, the p53-deficient 
      HCT116 cells showed less susceptibility to the HW1-mediated autophagic cell death 
      than the wild type cells, suggesting that JNK-mediated p53 phosphorylation 
      promotes the autophagic cell death. Our results suggest that DR5-stimulated JNK 
      activation and its consequent fluxes into the pro-autophagic signaling pathways 
      contribute to the autophagic cell death in cancer cells.
FAU - Park, Kyung-Jin
AU  - Park KJ
AD  - Dept. of Molecular Science and Technology, Ajou University, Yeongtong-Gu, Suwon, 
      Republic of Korea.
FAU - Lee, Seung-Hyun
AU  - Lee SH
FAU - Lee, Chang-Han
AU  - Lee CH
FAU - Jang, Ji-Young
AU  - Jang JY
FAU - Chung, Junho
AU  - Chung J
FAU - Kwon, Myung-Hee
AU  - Kwon MH
FAU - Kim, Yong-Sung
AU  - Kim YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090324
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Apoptosis Regulatory Proteins/*biosynthesis
MH  - *Autophagy
MH  - Beclin-1
MH  - Cell Line, Tumor
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Membrane Proteins/*biosynthesis
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-bcl-2/*metabolism
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/agonists
MH  - Tumor Suppressor Protein p53/*metabolism
MH  - Up-Regulation
EDAT- 2009/03/26 09:00
MHDA- 2009/05/16 09:00
CRDT- 2009/03/26 09:00
PHST- 2009/03/17 00:00 [received]
PHST- 2009/03/17 00:00 [accepted]
PHST- 2009/03/26 09:00 [entrez]
PHST- 2009/03/26 09:00 [pubmed]
PHST- 2009/05/16 09:00 [medline]
AID - S0006-291X(09)00573-7 [pii]
AID - 10.1016/j.bbrc.2009.03.095 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 May 15;382(4):726-9. doi: 
      10.1016/j.bbrc.2009.03.095. Epub 2009 Mar 24.