PMID- 19318515
OWN - NLM
STAT- MEDLINE
DCOM- 20090716
LR  - 20181201
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 296
IP  - 6
DP  - 2009 Jun
TI  - Muscle-specific differences in the response of mitochondrial proteins to beta-GPA 
      feeding: an evaluation of potential mechanisms.
PG  - E1400-8
LID - 10.1152/ajpendo.90913.2008 [doi]
AB  - Beta-Guanadinopropionic acid (beta-GPA) feeding leads to reductions in skeletal 
      muscle phosphagen concentrations and has been used as a tool with which to study 
      the effects of energy charge on skeletal muscle metabolism. Supplementing 
      standard rodent diets with beta-GPA leads to increases in mitochondrial enzyme 
      content in fast but not slow-twitch muscles from male rats. Given this apparent 
      discrepancy between muscle types we used beta-GPA feeding as a model to study 
      signaling pathways involved in mitochondrial biogenesis. We hypothesized that 
      beta-GPA feeding would result in a preferential activation of p38 MAPK and AMPK 
      signaling and reductions in RIP140 protein content in triceps but not soleus 
      muscle. Despite similar reductions in high-energy phosphate concentrations, 6 wk 
      of beta-GPA feeding led to increases in mitochondrial proteins in triceps but not 
      soleus muscles. Differences in the response of mitochondrial proteins to beta-GPA 
      feeding did not seem to be related to a differential activation of p38 MAPK and 
      AMPK signaling pathways or discrepancies in the induction of PPARgamma 
      coactivator (PGC)-1alpha and -1beta. The protein content and expression of the 
      nuclear corepressor RIP140 was reduced in triceps but not soleus muscle. 
      Collectively our results indicate that chronic reductions in high-energy 
      phosphates lead to the activation of p38 MAPK and AMPK signaling and increases in 
      the expression of PGC-1alpha and -1beta in both soleus and triceps muscles. The 
      lack of an effect of beta-GPA feeding on mitochondrial proteins in the soleus 
      muscles could be related to a fiber type-specific effect of beta-GPA on RIP140 
      protein content.
FAU - Williams, Deon B
AU  - Williams DB
AD  - Alberta Diabetes Institute, 4126C HRIF East, Univ. of Alberta, Edmonton, Alberta, 
      Canada T6G 2E1.
FAU - Sutherland, Lindsey N
AU  - Sutherland LN
FAU - Bomhof, Marc R
AU  - Bomhof MR
FAU - Basaraba, Susan A U
AU  - Basaraba SA
FAU - Thrush, A Brianne
AU  - Thrush AB
FAU - Dyck, David J
AU  - Dyck DJ
FAU - Field, Catherine J
AU  - Field CJ
FAU - Wright, David C
AU  - Wright DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090324
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (Guanidines)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nuclear Receptor Interacting Protein 1)
RN  - 0 (PPAR gamma)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, rat)
RN  - 0 (Propionates)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.4.3 (Adenylate Kinase)
RN  - UL1984YRKA (guanidinopropionic acid)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Adenylate Kinase/*metabolism
MH  - Animal Feed
MH  - Animals
MH  - Body Weight/drug effects/physiology
MH  - DNA, Mitochondrial/genetics
MH  - Energy Metabolism/drug effects/physiology
MH  - Guanidines/*pharmacology
MH  - Male
MH  - Mitochondrial Proteins/genetics/metabolism
MH  - Muscle Fibers, Fast-Twitch/drug effects/enzymology
MH  - Muscle Fibers, Slow-Twitch/drug effects/enzymology
MH  - Muscle, Skeletal/*cytology/*enzymology
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Nuclear Receptor Interacting Protein 1
MH  - PPAR gamma/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Propionates/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects/physiology
MH  - Transcription Factors/genetics/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2009/03/26 09:00
MHDA- 2009/07/17 09:00
CRDT- 2009/03/26 09:00
PHST- 2009/03/26 09:00 [entrez]
PHST- 2009/03/26 09:00 [pubmed]
PHST- 2009/07/17 09:00 [medline]
AID - 90913.2008 [pii]
AID - 10.1152/ajpendo.90913.2008 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2009 Jun;296(6):E1400-8. doi: 
      10.1152/ajpendo.90913.2008. Epub 2009 Mar 24.