PMID- 19319187
OWN - NLM
STAT- MEDLINE
DCOM- 20090701
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 3
DP  - 2009
TI  - Glutathione deficiency in cardiac patients is related to the functional status 
      and structural cardiac abnormalities.
PG  - e4871
LID - 10.1371/journal.pone.0004871 [doi]
LID - e4871
AB  - BACKGROUND: The tripeptide glutathione (L-gamma-glutamyl-cysteinyl-glycine) is 
      essential to cell survival, and deficiency in cardiac and systemic glutathione 
      relates to heart failure progression and cardiac remodelling in animal models. 
      Accordingly, we investigated cardiac and blood glutathione levels in patients of 
      different functional classes and with different structural heart diseases. 
      METHODS: Glutathione was measured using standard enzymatic recycling method in 
      venous blood samples obtained from 91 individuals, including 15 healthy 
      volunteers and 76 patients of New York Heart Association (NYHA) functional class 
      I to IV, undergoing cardiac surgery for coronary artery disease, aortic stenosis 
      or terminal cardiomyopathy. Glutathione was also quantified in right atrial 
      appendages obtained at the time of surgery. RESULTS: In atrial tissue, 
      glutathione was severely depleted (-58%) in NYHA class IV patients compared to 
      NYHA class I patients (P = 0.002). In patients with coronary artery disease, this 
      depletion was related to the severity of left ventricular dysfunction (P = 
      0.006). Compared to healthy controls, blood glutathione was decreased by 21% in 
      NYHA class I patients with structural cardiac disease (P<0.01), and by 40% in 
      symptomatic patients of NYHA class II to IV (P<0.0001). According to the 
      functional NYHA class, significant depletion in blood glutathione occurred before 
      detectable elevation in blood sTNFR1, a marker of symptomatic heart failure 
      severity, as shown by the exponential relationship between these two parameters 
      in the whole cohort of patients (r = 0.88). CONCLUSIONS: This study provides 
      evidence that cardiac and systemic glutathione deficiency is related to the 
      functional status and structural cardiac abnormalities of patients with cardiac 
      diseases. These data also suggest that blood glutathione test may be an 
      interesting new biomarker to detect asymptomatic patients with structural cardiac 
      abnormalities.
FAU - Damy, Thibaud
AU  - Damy T
AD  - Departement de Chirurgie Cardiaque, AP-HP, Groupe Hospitalier Henri-Mondor 
      Albert-Chenevier, Federation de Cardiologie, Creteil, France.
FAU - Kirsch, Matthias
AU  - Kirsch M
FAU - Khouzami, Lara
AU  - Khouzami L
FAU - Caramelle, Philippe
AU  - Caramelle P
FAU - Le Corvoisier, Philippe
AU  - Le Corvoisier P
FAU - Roudot-Thoraval, Francoise
AU  - Roudot-Thoraval F
FAU - Dubois-Rande, Jean-Luc
AU  - Dubois-Rande JL
FAU - Hittinger, Luc
AU  - Hittinger L
FAU - Pavoine, Catherine
AU  - Pavoine C
FAU - Pecker, Francoise
AU  - Pecker F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090325
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cardiovascular Diseases/*diagnosis/surgery
MH  - Case-Control Studies
MH  - Female
MH  - Glutathione/analysis/*deficiency
MH  - Heart Atria/*chemistry
MH  - Heart Defects, Congenital/*diagnosis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Myocardium/chemistry
MH  - *Severity of Illness Index
MH  - Ventricular Dysfunction, Left
PMC - PMC2655715
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/03/26 09:00
MHDA- 2009/07/02 09:00
PMCR- 2009/03/25
CRDT- 2009/03/26 09:00
PHST- 2008/12/02 00:00 [received]
PHST- 2009/02/02 00:00 [accepted]
PHST- 2009/03/26 09:00 [entrez]
PHST- 2009/03/26 09:00 [pubmed]
PHST- 2009/07/02 09:00 [medline]
PHST- 2009/03/25 00:00 [pmc-release]
AID - 08-PONE-RA-07567R1 [pii]
AID - 10.1371/journal.pone.0004871 [doi]
PST - ppublish
SO  - PLoS One. 2009;4(3):e4871. doi: 10.1371/journal.pone.0004871. Epub 2009 Mar 25.