PMID- 19322783
OWN - NLM
STAT- MEDLINE
DCOM- 20090720
LR  - 20121115
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 9
IP  - 8
DP  - 2009 Apr
TI  - Detection of allergen specific immunoglobulins by microarrays coupled to 
      microfluidics.
PG  - 2098-107
LID - 10.1002/pmic.200800651 [doi]
AB  - Allergen microarrays are under development for a component-resolved diagnosis of 
      Type I (IgE-mediated) allergies. Here we report an improved microarray coupled to 
      microfluidics for the detection of allergen specific immunoglobulin E (IgE). The 
      signal intensity for IgE detection in serum has been improved by using glass 
      slides coated with a novel poly[DMA-co-NAS] brush copolymer which is able to 
      immobilize allergens in their native conformation and by carrying out the 
      incubation step in dynamic conditions. The assay, fully automated, was performed 
      in a microcell, using a software-controlled fluidic processor, to bring assay 
      reagents on the surface of the array. Microfluidics turns the binding between 
      serum immunoglobulins and immobilized allergens from a diffusion-limited to a 
      kinetic-limited process by ensuring an efficient mixing of serum samples on the 
      surface of the microarray. As a result of this, the binding of high affinity IgE 
      antibodies is enhanced whereas that of low affinity IgG antibodies, which are 
      present at higher concentration, is impaired paving the way to more accurate and 
      sensitive results.
FAU - Cretich, Marina
AU  - Cretich M
AD  - Istituto di Chimica del Riconoscimento Molecolare (ICRM), CNR, Milano, Italy. 
      marina.cretich@icrm.cnr.it
FAU - Di Carlo, Gabriele
AU  - Di Carlo G
FAU - Giudici, Cinzia
AU  - Giudici C
FAU - Pokoj, Sven
AU  - Pokoj S
FAU - Lauer, Iris
AU  - Lauer I
FAU - Scheurer, Stephan
AU  - Scheurer S
FAU - Chiari, Marcella
AU  - Chiari M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Acrylamides)
RN  - 0 (Acrylates)
RN  - 0 (Antigens, Plant)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Immunoglobulin G)
RN  - 0 (N,N-acryloyloxysuccinimide)
RN  - 0 (Succinimides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - AS46JK7Q6I (N,N-dimethylacrylamide)
SB  - IM
MH  - Acrylamides/chemistry/metabolism
MH  - Acrylates/chemistry/metabolism
MH  - Antibody Affinity
MH  - Antigens, Plant/*chemistry/metabolism
MH  - Coated Materials, Biocompatible/chemistry/metabolism
MH  - Humans
MH  - Hypersensitivity/blood/*diagnosis
MH  - Immunoglobulin E/blood/*chemistry
MH  - Immunoglobulin G/blood/chemistry
MH  - Luminescent Measurements
MH  - Microfluidics/*methods
MH  - Protein Array Analysis/*methods
MH  - Sensitivity and Specificity
MH  - Succinimides/chemistry/metabolism
EDAT- 2009/03/27 09:00
MHDA- 2009/07/21 09:00
CRDT- 2009/03/27 09:00
PHST- 2009/03/27 09:00 [entrez]
PHST- 2009/03/27 09:00 [pubmed]
PHST- 2009/07/21 09:00 [medline]
AID - 10.1002/pmic.200800651 [doi]
PST - ppublish
SO  - Proteomics. 2009 Apr;9(8):2098-107. doi: 10.1002/pmic.200800651.