PMID- 19323017
OWN - NLM
STAT- MEDLINE
DCOM- 20090623
LR  - 20090327
IS  - 0125-1562 (Print)
IS  - 0125-1562 (Linking)
VI  - 40
IP  - 2
DP  - 2009 Mar
TI  - Development of a comprehensive red blood cell enzymopathy laboratory in Thailand: 
      the study of normal activity in eight erythroenzymes in Thais.
PG  - 317-26
AB  - In order to provide a reference range for normal red blood cell enzyme activities 
      in Thai, we analyzed data from 113 healthy non-anemic Thai people (55 males and 
      58 females) age 1-42 years, who all had a normal pattern of hemoglobin typing 
      (HbA and HbA2 less than 3.5%). Hematological analysis was performed using an 
      automated cell counter and the hemoglobin studies were carried out by low 
      pressure liquid chromatography. Owing to a high frequency of alpha-thalassemia in 
      Thailand, cases with an MCV < 75 fl were excluded from the study since these 
      cases were likely to be heterozygotes for alpha0-thalassemia. Cases with 
      reticulocytes > 2.5% were excluded from the study since reticulocytes have a 
      higher enzyme activity than mature erythrocytes. Cases with abnormal red blood 
      cell morphology, such as spherocytes and ovalocytes, were also excluded. These 
      criteria were applied to select "normal" controls for our analysis. We assayed 
      eight red blood cell enzyme activities in normal subjects: glucose-6-phosphate 
      dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), pyruvate kinase 
      (PK), hexokinase (HK), glucose phosphate isomerase (GPI), phosphofructokinase 
      (PFK), aldolase (ALD) and phosphoglycerate kinase (PGK). The mean normal ranges 
      (+/- SD) for G6PD, 6PGD, PK, HK, GPI, PFK, ALD and PGK were 12.7 (+/-2.2), 10.7 
      (+/-1.3), 18.5 (+/-4.0), 1.5 (+/-0.4), 80.5 (+/-11.8), 11.8 (+/-2.1), 4.5 
      (+/-1.6) and 370 (+/-43) IU/gHb, respectively. Age-dependent differences for the 
      reference values for these enzyme activities were summarized. All red blood cell 
      enzyme activities were highest during the early childhood period and slightly 
      lower in the adult period. These values will be of clinically useful for future 
      reference.
FAU - Tachavanich, Kalaya
AU  - Tachavanich K
AD  - Hematology/Oncology Division, Department of Pediatrics, Faculty of Medicine 
      Siriraj Hospital, Mahidol University, Bangkok, Thailand. kalayata@yahoo.com
FAU - Viprakasit, Vip
AU  - Viprakasit V
FAU - Pung-Amritt, Parichat
AU  - Pung-Amritt P
FAU - Veerakul, Gavivann
AU  - Veerakul G
FAU - Chansing, Kochpinchorn
AU  - Chansing K
FAU - Tanphaichitr, Voravarn S
AU  - Tanphaichitr VS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Southeast Asian J Trop Med Public Health
JT  - The Southeast Asian journal of tropical medicine and public health
JID - 0266303
RN  - EC 1.1.- (Carbohydrate Dehydrogenases)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Analysis of Variance
MH  - Carbohydrate Dehydrogenases/blood
MH  - Child
MH  - Child, Preschool
MH  - Chromatography, Liquid
MH  - Electrophoresis
MH  - Erythrocyte Indices/*physiology
MH  - Erythrocytes/*enzymology
MH  - Female
MH  - Fructose-Bisphosphate Aldolase/blood
MH  - Glucose-6-Phosphate Isomerase/blood
MH  - Humans
MH  - Infant
MH  - Male
MH  - Phosphotransferases/blood
MH  - Reference Values
MH  - Thailand
MH  - Young Adult
EDAT- 2009/03/28 09:00
MHDA- 2009/06/24 09:00
CRDT- 2009/03/28 09:00
PHST- 2009/03/28 09:00 [entrez]
PHST- 2009/03/28 09:00 [pubmed]
PHST- 2009/06/24 09:00 [medline]
PST - ppublish
SO  - Southeast Asian J Trop Med Public Health. 2009 Mar;40(2):317-26.