PMID- 1932375
OWN - NLM
STAT- MEDLINE
DCOM- 19911202
LR  - 20171116
IS  - 1056-5477 (Print)
IS  - 1056-5477 (Linking)
VI  - 10
IP  - 4
DP  - 1991 Aug
TI  - Lymphocytes infiltrating ovarian malignant ascites: modulation of IL-2-induced 
      proliferation by IL-4 and of selective increase in CD8+ T cells by TNF-alpha.
PG  - 307-15
AB  - Lymphocytes infiltrating ovarian malignant ascites (TALs) were propagated in 
      vitro in the presence of low (20-50 U/ml) concentrations of recombinant 
      interleukin-2 (rIL-2) and moderate concentrations of interleukin-4 (IL-4) and 
      tumor necrosis factor type alpha (TNF-alpha). Neither IL-4 nor TNF-alpha alone 
      induced proliferation of ovarian TALs, but IL-4 and TNF-alpha synergized with 
      IL-2 in enhancing the proliferation of T cells from cultures and inducing a more 
      specific pattern of cytotoxic responses of ovarian TAL. IL-4 enhanced the 
      proliferation of TAL cultures in the induction phase, but did not synergize with 
      IL-2 in enhancing the proliferation and cytotoxicity of an established autologous 
      tumor-specific CD8+/CD4- cytotoxic T lymphocyte (CTL) line. In contrast, 
      TNF-alpha-induced preferential expansion of CD8+ CTL in TAL cultures where 
      CD3+/CD4+ cells were initially in excess and of an established autologous 
      tumor-specific CD8+ CTL line. This expansion resulted in increased levels of 
      cytoxicity against autologous tumor cells. Amplification of IL-2 growth-promoting 
      effects and selective enrichment in T cell subsets by IL-4 and TNF-alpha may 
      therefore be useful in cellular adoptive immunotherapy using tumor-specific T 
      cells.
FAU - Ioannides, C G
AU  - Ioannides CG
AD  - Department of Gynecology, University of Texas, M.D. Anderson Cancer Center, 
      Houston 77030.
FAU - Rashed, S
AU  - Rashed S
FAU - Fisk, B
AU  - Fisk B
FAU - Fan, D
AU  - Fan D
FAU - Itoh, K
AU  - Itoh K
FAU - Freedman, R S
AU  - Freedman RS
LA  - eng
GR  - RR-5511/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lymphokine Cytokine Res
JT  - Lymphokine and cytokine research
JID - 9107882
RN  - 0 (CD8 Antigens)
RN  - 0 (Interleukin-2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Adenocarcinoma/*immunology
MH  - Ascites/immunology
MH  - CD8 Antigens/analysis
MH  - Cytotoxicity, Immunologic/drug effects
MH  - Female
MH  - Humans
MH  - Interleukin-2/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Lymphocyte Activation/drug effects
MH  - Lymphocytes, Tumor-Infiltrating/*immunology
MH  - Ovarian Neoplasms/*immunology
MH  - T-Lymphocyte Subsets/*immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PST - ppublish
SO  - Lymphokine Cytokine Res. 1991 Aug;10(4):307-15.