PMID- 19323903
OWN - NLM
STAT- MEDLINE
DCOM- 20090522
LR  - 20090327
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 122
IP  - 5
DP  - 2009 Mar 5
TI  - Different plasma levels of interleukins and chemokines: comparison between 
      children and adults with AIDS in China.
PG  - 530-5
AB  - BACKGROUND: The immunological differences between children and adults with AIDS 
      in China are not well documented. Th1/Th2 cytokines and chemokines are two types 
      of immune factors intimately involved in disease progression of HIV-1 infection. 
      This study aimed to identify changes in plasma levels of Th1/Th2 cytokines 
      inerleukin (IL)-18, IL-16, IL-10 and chemokines regulated on activation, normal T 
      cell expressed and secreted (RANTES), stromal cell-derived factor-1 (SDF-1) and 
      monocyte chemoattractant protein-1 (MCP-1) in HIV-1-infected children and adults 
      in China. METHODS: Seventy-five children with AIDS and 35 adult AIDS patients 
      were recruited and clinical data were collected. CD4(+) T lymphocyte counts were 
      measured by flow cytometery and plasma HIV RNA levels were measured by 
      quantitative RT-PCR. Plasma levels of IL-18, IL-10, IL-16, RANTES, MCP-1, 
      SDF-1alpha and SDF-1beta were quantified by enzyme-linked immunosorbent assay. 
      The levels of beta2-microglobulin (beta2-MG) and soluble Fas (sFas) were measured 
      to validate the level of humoral and cellular immune activation. RESULTS: The 
      mean levels of all cytokines in pediatric and adult AIDS patients were 
      significantly higher than in their healthy controls (P < 0.01). The mean levels 
      of these cytokines were higher in pediatric patients than in adult patients (P < 
      0.05, except for SDF-1alpha and beta2-MG). Some of the cytokine levels in 
      patients younger than 6 years old was higher than in older children and adults 
      with AIDS (IL-10, IL-18, SDF-1alpha, MCP, RANTES and sFas, P < 0.05). Levels of 
      IL-18, IL-10, RANTES and beta2-MG of pediatric patients increased as the levels 
      of viral load increased (P < 0.05). CONCLUSIONS: Abnormal immune activation can 
      be measured in Chinese pediatric and adult patients with AIDS, and is higher in 
      children than in adult patients. The cytokines levels coincide with disease 
      progression of AIDS, but have no direct relationship with total CD4(+) T cell 
      count.
FAU - Jin, Chang-zhong
AU  - Jin CZ
AD  - First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 
      China.
FAU - Zhao, Yan
AU  - Zhao Y
FAU - Zhang, Fu-jie
AU  - Zhang FJ
FAU - Yao, Hang-ping
AU  - Yao HP
FAU - Wu, Ling-jiao
AU  - Wu LJ
FAU - Zhao, Hong-xin
AU  - Zhao HX
FAU - Wei, Hong-shan
AU  - Wei HS
FAU - Wu, Nan-ping
AU  - Wu NP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-16)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukins)
RN  - 0 (RNA, Viral)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*blood/virology
MH  - Adult
MH  - Age Distribution
MH  - Aged
MH  - Chemokine CCL2/blood
MH  - Chemokine CCL5/blood
MH  - Chemokine CXCL12/blood
MH  - Chemokines/*blood
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - HIV-1/genetics
MH  - Humans
MH  - Interleukin-10/blood
MH  - Interleukin-16/blood
MH  - Interleukin-18/blood
MH  - Interleukins/*blood
MH  - Male
MH  - Middle Aged
MH  - RNA, Viral/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Viral Load
EDAT- 2009/03/28 09:00
MHDA- 2009/05/23 09:00
CRDT- 2009/03/28 09:00
PHST- 2009/03/28 09:00 [entrez]
PHST- 2009/03/28 09:00 [pubmed]
PHST- 2009/05/23 09:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2009 Mar 5;122(5):530-5.