PMID- 19324971
OWN - NLM
STAT- MEDLINE
DCOM- 20091012
LR  - 20211020
IS  - 1944-9917 (Electronic)
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 23
IP  - 7
DP  - 2009 Jul
TI  - Estrogen receptor beta is required for optimal cAMP production in mouse granulosa 
      cells.
PG  - 955-65
LID - 10.1210/me.2008-0213 [doi]
AB  - Granulosa cells of preovulatory follicles differentiate in response to FSH, and 
      this differentiation is augmented by estradiol. We have previously shown that 
      FSH-mediated granulosa cell differentiation requires functional estrogen 
      receptor-beta (ERbeta) by demonstrating that the granulosa cells of ERbeta(-/-) 
      FSH-treated mice are unable to maximally induce expression of the LH receptor (an 
      indicator of granulosa cell differentiation) compared with ERbeta(+/+) controls. 
      As a result, FSH-primed ERbeta(-/-) granulosa cells exhibit a reduced response to 
      a subsequent ovulatory dose of LH. In this study, we further characterized the 
      attenuated response of ERbeta(-/-) granulosa cells to stimulation by LH and FSH 
      using isolated mouse granulosa cells and primary granulosa cell cultures. We 
      observed a 50% reduction in cAMP levels in cultured ERbeta(-/-) granulosa cells 
      exposed to LH compared with ERbeta(+/+) controls. We also observed an attenuated 
      genomic response in granulosa cells isolated from FSH-primed ERbeta(-/-) mice 
      compared with ERbeta(+/+) controls. Our data indicate that this attenuated 
      response may result from inadequate levels of cAMP, because cAMP levels in 
      cultured ERbeta(-/-) granulosa cells exposed to forskolin were approximately 50% 
      lower than in ERbeta(+/+) granulosa cells. Phosphorylation of cAMP regulatory 
      element binding protein, an indicator of protein kinase A activity, was also 
      reduced in FSH-treated ERbeta(-/-) granulosa cells compared with ERbeta(+/+) 
      controls. These are the first data to indicate that ERbeta plays a role in the 
      induction of the cAMP pathway in mouse granulosa cells and that disruption of 
      proper ERbeta signaling associated with this pathway may cause negative effects 
      on ovulation and fertility.
FAU - Deroo, Bonnie J
AU  - Deroo BJ
AD  - Receptor Biology Section, National Institute of Environmental Health Sciences, 
      National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
FAU - Rodriguez, Karina F
AU  - Rodriguez KF
FAU - Couse, John F
AU  - Couse JF
FAU - Hamilton, Katherine J
AU  - Hamilton KJ
FAU - Collins, Jennifer B
AU  - Collins JB
FAU - Grissom, Sherry F
AU  - Grissom SF
FAU - Korach, Kenneth S
AU  - Korach KS
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090326
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Receptors, LH)
RN  - 1F7A44V6OU (Colforsin)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*metabolism
MH  - Down-Regulation/drug effects/genetics
MH  - Estrogen Receptor beta/genetics/metabolism/*physiology
MH  - Female
MH  - Fertility/genetics
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Luteinizing Hormone/pharmacology
MH  - Mice
MH  - Mice, Knockout
MH  - Ovulation/genetics/metabolism
MH  - Receptors, LH/genetics/metabolism
PMC - PMC2703605
EDAT- 2009/03/28 09:00
MHDA- 2009/10/13 06:00
PMCR- 2010/07/01
CRDT- 2009/03/28 09:00
PHST- 2009/03/28 09:00 [entrez]
PHST- 2009/03/28 09:00 [pubmed]
PHST- 2009/10/13 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - me.2008-0213 [pii]
AID - 4454 [pii]
AID - 10.1210/me.2008-0213 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2009 Jul;23(7):955-65. doi: 10.1210/me.2008-0213. Epub 2009 Mar 
      26.