PMID- 19325217
OWN - NLM
STAT- MEDLINE
DCOM- 20090812
LR  - 20190608
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 55
IP  - 3
DP  - 2009 Jun
TI  - Agrin pathway is controlled by leukemia inhibitory factor (LIF) in murine 
      implantation.
PG  - 293-8
AB  - Agrin is the heparan sulfate proteoglycan (HSPG) that is well known as the 
      molecule that aggregates acetylcholine receptor (AChR) through muscle specific 
      kinase (MuSK) and rapsyn at neuromuscular junctions. HSPGs are spatiotemporally 
      expressed in embryonic and maternal tissues during implantation. The present 
      study examined the role of agrin in the mouse embryo using leukemia inhibitory 
      factor (LIF)-deficient mice, which show complete sterility. Agrin was detected 
      widely in the cytoplasm of uterine luminal epithelial cells at the third day of 
      pregnancy (Day 3) and Day 4. At Day 5, agrin moved to the apical surface of the 
      luminal epithelium. This migration was not found in LIF-deficient mice. AChR was 
      also found in the apical surface of the uterine epithelium at Day 4 and Day 5 in 
      normal mice. LIF-deficient mice did not show this pattern of expression. Only 
      nAChR b1 subunit mRNA was increased at Day 5 in normal mice. Furthermore, 
      acetylcholinesterase was active in the uterine stroma of normal mice throughout 
      the implantation period and was exclusively active in the uterine epithelium at 
      Day 4. Taken together, agrin signaling was activated in the uterus during embryo 
      implantation in the mice. Here, we suggest that the agrin pathway is involved in 
      closure of the uterine epithelium toward placentation.
FAU - Terakawa, Jumpei
AU  - Terakawa J
AD  - Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University.
FAU - Hondo, Eiichi
AU  - Hondo E
FAU - Sugiyama, Makoto
AU  - Sugiyama M
FAU - Wakitani, Shoichi
AU  - Wakitani S
FAU - Stewart, Colin L
AU  - Stewart CL
FAU - Kiso, Yasuo
AU  - Kiso Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090326
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (Agrin)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Receptors, Cholinergic)
SB  - IM
MH  - Agrin/genetics/metabolism/*physiology
MH  - Animals
MH  - Embryo Implantation/genetics/*physiology
MH  - Female
MH  - Leukemia Inhibitory Factor/genetics/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Placentation/genetics/physiology
MH  - Pregnancy
MH  - Receptors, Cholinergic/genetics/metabolism
MH  - Signal Transduction/genetics/physiology
MH  - Uterus/metabolism
EDAT- 2009/03/28 09:00
MHDA- 2009/08/13 09:00
CRDT- 2009/03/28 09:00
PHST- 2009/03/28 09:00 [entrez]
PHST- 2009/03/28 09:00 [pubmed]
PHST- 2009/08/13 09:00 [medline]
AID - JST.JSTAGE/jrd/20162 [pii]
AID - 10.1262/jrd.20162 [doi]
PST - ppublish
SO  - J Reprod Dev. 2009 Jun;55(3):293-8. doi: 10.1262/jrd.20162. Epub 2009 Mar 26.