PMID- 19328877
OWN - NLM
STAT- MEDLINE
DCOM- 20090601
LR  - 20090511
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 26
IP  - 5
DP  - 2009 May
TI  - Effects of serum on phagocytic activity and proteomic analysis of tilapia 
      (Oreochromis mossambicus) serum after acute osmotic stress.
PG  - 760-7
LID - 10.1016/j.fsi.2009.03.005 [doi]
AB  - The objective of the study was to analyze the effect of serum from freshwater 
      (FW) exposed tilapia or from 25 ppt seawater (SW) exposed tilapia on the ability 
      to mediate the phagocytic activity of tilapia phagocytes. To analyze the 
      phagocytic activity, head kidney (HK) and spleen leukocytes were tested in 300 or 
      500 mOsm medium using three different treatment groups (a) control, (b) addition 
      of 25% serum from freshwater (FW) exposed tilapia, and (c) addition of 25% of 
      serum from 25 ppt seawater (SW) exposed tilapia. HK leukocytes cultured in 300 
      and 500 mOsm media for 4 h showed an increase of phagocytic ability in the 
      control group as compared to the addition of serum from either FW or SW exposed 
      tilapia. HK leukocytes exposed to 500 mOsm medium showed a higher phagocytic 
      ability than those leukocytes exposed to 300 mOsm medium in each corresponding 
      group. Concurrently, spleen leukocytes in the control group showed a higher 
      phagocytic ability than those leukocytes with the addition of serum from FW or SW 
      exposed tilapia. As compared to spleen leukocytes cultured in 300 mOsm medium, 
      leukocytes cultured in 500 mOsm medium showed an increase of phagocytic ability 
      within their respective group. To further investigate the observed phenomenon, 
      2D-gel electrophoresis was performed for analyzing the differentially expressed 
      proteins in serum that was thought to influence the phagocytic ability. 
      Up-regulated serum proteins in SW exposed tilapia contained complement C3 
      protein, NADH dehydrogenase (Ubiquinone) Fe-S protein 3, Mg(2+)-dependent neutral 
      sphingomyelinase, Semaphorins, and Caspase 3. Taken together these results 
      suggest that addition of serum decreased the phagocytic activity in HK and spleen 
      leukocytes in vitro, furthermore, induced proteins semaphorin, complement C3, 
      Mg(2+)-dependent neutral sphingomyelinase, and Caspase 3 are up-regulated in the 
      serum, which might have decreased the phagocytic activity upon exposure to 
      hyperosmotic solutions.
FAU - Kumar, V Bharath
AU  - Kumar VB
AD  - Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
FAU - Jiang, Ing-Fong
AU  - Jiang IF
FAU - Yang, Hsueh-Hui
AU  - Yang HH
FAU - Weng, Ching-Feng
AU  - Weng CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090327
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Blood Proteins)
SB  - IM
MH  - Animals
MH  - Blood Proteins/isolation & purification/*metabolism
MH  - *Fresh Water
MH  - Leukocytes/immunology
MH  - Phagocytosis/*physiology
MH  - Proteomics
MH  - *Seawater
MH  - Spleen/*immunology
MH  - Stress, Physiological/*immunology
MH  - Tilapia/blood/*immunology/physiology
EDAT- 2009/03/31 09:00
MHDA- 2009/06/02 09:00
CRDT- 2009/03/31 09:00
PHST- 2008/11/19 00:00 [received]
PHST- 2009/03/05 00:00 [revised]
PHST- 2009/03/08 00:00 [accepted]
PHST- 2009/03/31 09:00 [entrez]
PHST- 2009/03/31 09:00 [pubmed]
PHST- 2009/06/02 09:00 [medline]
AID - S1050-4648(09)00082-5 [pii]
AID - 10.1016/j.fsi.2009.03.005 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2009 May;26(5):760-7. doi: 10.1016/j.fsi.2009.03.005. 
      Epub 2009 Mar 27.