PMID- 1933051
OWN - NLM
STAT- MEDLINE
DCOM- 19911209
LR  - 20141120
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 8
IP  - 2
DP  - 1991 Aug
TI  - Cytotoxic and proliferative functions of T lymphocyte clones derived very shortly 
      after allogeneic bone marrow transplantation.
PG  - 113-8
AB  - In a search for mechanisms of potential graft-versus-leukaemia (GVL) activity 
      after allogeneic bone marrow transplantation (BMT), peripheral lymphocytes from 
      five patients (four chronic myeloid leukaemia, one acute lymphoblastic leukaemia) 
      24-39 days post-transplant were precultured with pretransplant host leukaemia 
      cells and then cloned by limiting dilution with interleukin-2 (IL-2). Clones 
      obtained were exclusively CD3+ CD56-, carried the alpha/beta form of the T cell 
      receptor for antigen, and were mostly (88% of 138) CD4+. None of 143 clones, 
      including CD8+ clones, convincingly lysed host pretransplant cells, although 35 
      (24.5%) manifested lytic potential in lectin-mediated cytotoxicity assays. 
      Measuring the proliferative responses of 118 of these clones in the presence of 
      exogenous IL-2 revealed that a small number of clones reacted more strongly to 
      host leukaemia than to unrelated leukaemias or B lymphoblastoid cell lines. In 
      the two cases tested, the donor's untransplanted lymphocytes cloned under the 
      same conditions as post-transplant cells did not generate any clones reacting 
      preferentially with host leukaemia cells. These results may suggest that some T 
      cells appearing shortly after allogeneic BMT could potentially mediate 
      anti-leukaemia activity not associated with cytolysis of target cells.
FAU - Hamann, W
AU  - Hamann W
AD  - Second Department of Internal Medicine, Medical Clinic of the University of 
      Tubingen, Germany.
FAU - Bruserud, O
AU  - Bruserud O
FAU - Patel, S
AU  - Patel S
FAU - Schmidt, H
AU  - Schmidt H
FAU - Ehninger, G
AU  - Ehninger G
FAU - Pawelec, G
AU  - Pawelec G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
SB  - IM
MH  - Antigens, Differentiation, T-Lymphocyte
MH  - Bone Marrow Transplantation/*immunology
MH  - Clone Cells/immunology
MH  - Cytotoxicity, Immunologic
MH  - Graft vs Host Disease/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/surgery
MH  - Lymphocyte Activation
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/surgery
MH  - T-Lymphocytes/*immunology
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PST - ppublish
SO  - Bone Marrow Transplant. 1991 Aug;8(2):113-8.