PMID- 19331145
OWN - NLM
STAT- MEDLINE
DCOM- 20090416
LR  - 20191210
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 29
IP  - 1
DP  - 2009 Jan
TI  - Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase, and vitamin D receptor 
      mRNA in normal and malignant breast tissue.
PG  - 155-7
AB  - Vitamin D has anti-proliferative and proapoptotic effects on several cell types, 
      including breast cancer cells. There have been no studies quantifying the 
      expression of the enzyme 25-hydroxyvitamin D-1-alpha-hydroxylase (1alphaOHase), 
      which converts 25-hydroxyvitamin D to its active metabolite, in breast tissue. We 
      performed real-time RT-PCR to quantity 1alphaOHase and vitamin D receptor (VDR) 
      mRNA in samples of breast cancer, adjacent non-cancerous tissue and normal breast 
      tissue. 1alphaOHase and VDR mRNA were universally expressed, however, 1alphaOHase 
      expression was significantly down-regulated in adjacent non-cancerous tissue from 
      women with breast cancer in comparison to individuals without cancer. VDR was 
      also up-regulated in breast tumours. The vitamin D axis expression in the breast 
      suggests a role for its actions in normal tissue homeostasis and breast cancer 
      pathogenesis. The decreased expression of 1alphaOHase in normal tissue from women 
      with breast cancer may be important in their predisposition to the development of 
      cancer.
FAU - McCarthy, Kathryn
AU  - McCarthy K
AD  - Section of Endocrine Oncology, Department of Endocrinology, Barts and The London, 
      Queen Mary School of Medicine, St. Bartholomew's Hospital, London EC1A 6BT, UK. 
      kathrynmccarthy@doctors.org.uk
FAU - Laban, Christiana
AU  - Laban C
FAU - Bustin, Stephen A
AU  - Bustin SA
FAU - Ogunkolade, William
AU  - Ogunkolade W
FAU - Khalaf, Sahira
AU  - Khalaf S
FAU - Carpenter, Rob
AU  - Carpenter R
FAU - Jenkins, Paul J
AU  - Jenkins PJ
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Calcitriol)
RN  - EC 1.14.15.18 (25-Hydroxyvitamin D3 1-alpha-Hydroxylase)
SB  - IM
EIN - Anticancer Res. 2009 Oct;29(10):4315
MH  - 25-Hydroxyvitamin D3 1-alpha-Hydroxylase/*biosynthesis/genetics
MH  - Breast/metabolism
MH  - Breast Neoplasms/enzymology/genetics/*metabolism
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptors, Calcitriol/*biosynthesis/genetics
EDAT- 2009/04/01 09:00
MHDA- 2009/04/17 09:00
CRDT- 2009/04/01 09:00
PHST- 2009/04/01 09:00 [entrez]
PHST- 2009/04/01 09:00 [pubmed]
PHST- 2009/04/17 09:00 [medline]
PST - ppublish
SO  - Anticancer Res. 2009 Jan;29(1):155-7.