PMID- 19332545
OWN - NLM
STAT- MEDLINE
DCOM- 20090806
LR  - 20211020
IS  - 0021-9258 (Print)
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Linking)
VI  - 284
IP  - 26
DP  - 2009 Jun 26
TI  - Abscisic acid released by human monocytes activates monocytes and vascular smooth 
      muscle cell responses involved in atherogenesis.
PG  - 17808-18
LID - 10.1074/jbc.M809546200 [doi]
AB  - Abscisic acid (ABA) is a phytohormone recently identified as a new endogenous 
      pro-inflammatory hormone in human granulocytes. Here we report the functional 
      activation of human monocytes and vascular smooth muscle cells by ABA. Incubation 
      of monocytes with ABA evokes an intracellular Ca2+ rise through the second 
      messenger cyclic ADP-ribose, leading to NF-kappaB activation and consequent 
      increase of cyclooxygenase-2 expression and prostaglandin E2 production and 
      enhanced release of MCP-1 (monocyte chemoattractant protein-1) and of 
      metalloprotease-9, all events reportedly involved in atherogenesis. Moreover, 
      monocytes release ABA when exposed to thrombin-activated platelets, a condition 
      occurring at the injured vascular endothelium; monocyte-derived ABA behaves as an 
      autocrine and paracrine pro-inflammatory hormone-stimulating monocyte migration 
      and MCP-1 release, as well as vascular smooth muscle cells migration and 
      proliferation. These results, and the presence of ABA in human arterial plaques 
      at a 10-fold higher concentration compared with normal arterial tissue, identify 
      ABA as a new signal molecule involved in the development of atherosclerosis and 
      suggest a possible new target for anti-atherosclerotic therapy.
FAU - Magnone, Mirko
AU  - Magnone M
AD  - Department of Experimental Medicine, Section of Biochemistry and Center of 
      Excellence for Biomedical Research, University of Genova, and Vascular and 
      Endovascular Surgery Unit, San Martino Hospital, Viale Benedetto XV 1, 16132 
      Genova, Italy.
FAU - Bruzzone, Santina
AU  - Bruzzone S
FAU - Guida, Lucrezia
AU  - Guida L
FAU - Damonte, Gianluca
AU  - Damonte G
FAU - Millo, Enrico
AU  - Millo E
FAU - Scarfi, Sonia
AU  - Scarfi S
FAU - Usai, Cesare
AU  - Usai C
FAU - Sturla, Laura
AU  - Sturla L
FAU - Palombo, Domenico
AU  - Palombo D
FAU - De Flora, Antonio
AU  - De Flora A
FAU - Zocchi, Elena
AU  - Zocchi E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090330
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Hemostatics)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (RNA, Messenger)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 3.4.21.5 (Thrombin)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Abscisic Acid/*pharmacology
MH  - Aorta/cytology/metabolism
MH  - Atherosclerosis/*metabolism/pathology
MH  - Blotting, Western
MH  - Calcium/metabolism
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Cyclooxygenase 2/metabolism
MH  - Dinoprostone/metabolism
MH  - Hemostatics/pharmacology
MH  - Humans
MH  - Monocytes/*drug effects/metabolism
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism
MH  - NF-kappa B/metabolism
MH  - Plant Growth Regulators/*pharmacology
MH  - Platelet Activation/drug effects
MH  - Protein Transport
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Second Messenger Systems
MH  - Thrombin/pharmacology
PMC - PMC2719419
EDAT- 2009/04/01 09:00
MHDA- 2009/08/07 09:00
PMCR- 2010/06/26
CRDT- 2009/04/01 09:00
PHST- 2009/04/01 09:00 [entrez]
PHST- 2009/04/01 09:00 [pubmed]
PHST- 2009/08/07 09:00 [medline]
PHST- 2010/06/26 00:00 [pmc-release]
AID - S0021-9258(19)82063-X [pii]
AID - M809546200 [pii]
AID - 10.1074/jbc.M809546200 [doi]
PST - ppublish
SO  - J Biol Chem. 2009 Jun 26;284(26):17808-18. doi: 10.1074/jbc.M809546200. Epub 2009 
      Mar 30.