PMID- 19332649
OWN - NLM
STAT- MEDLINE
DCOM- 20090903
LR  - 20131121
IS  - 1096-0929 (Electronic)
IS  - 1096-0929 (Linking)
VI  - 109
IP  - 2
DP  - 2009 Jun
TI  - Is toluene diamine a sensitizer and is there cross-reactivity between toluene 
      diamine and toluene diisocyanate?
PG  - 256-64
LID - 10.1093/toxsci/kfp065 [doi]
AB  - Toluene diamine (TDA) is formed when toluene diisocyanate (TDI), a potent 
      sensitizer, comes in contact with an aqueous environment. The sensitizing 
      capacity of TDA and the cross-reactivity between TDI and TDA are unknown. TDA 
      (5-25%) and TDI (0.3%), dissolved in acetone/olive oil (AOO) (4:1) were tested in 
      the mouse local lymph node assay (LLNA). To determine the capacity of TDA to 
      elicit an asthmatic response and to determine the cross-reaction with TDI, a 
      locally developed experimental mouse model of chemical-induced asthma was used. 
      On days 1 and 8, BALB/c mice received 20 microl of TDI (0.3%), TDA (20%), or AOO 
      (4:1) on each ear. On day 15, they received an intranasal instillation of TDI 
      (0.1%), TDA (0.5%) or AOO (3:2). The EC(3) of TDA in the LLNA is 19%. In the 
      model of chemical-induced asthma, TDI induced a ventilatory response [increased 
      Penh after challenge; increased airway hyperreactivity (AHR)], inflammatory 
      changes (bronchoalveolar lavage neutrophils), and immunological changes 
      (increased CD19(+) lymphocytes, IL-4 and total serum IgE), whereas TDA did not 
      show any of these responses. Mice sensitized with TDI and challenged with TDA 
      also did not show any airway or inflammatory response, although they had 
      increased levels of total serum IgE. Mice sensitized with TDA and challenged with 
      TDI did not show any response. According to the classification of sensitizers in 
      the LLNA, TDA is a weak dermal sensitizer. In the experimental mouse model of 
      chemical-induced asthma, TDA does not act as a respiratory sensitizer, at the 
      concentration used. No cross-reactivity between TDI and TDA was found.
FAU - Vanoirbeek, Jeroen A J
AU  - Vanoirbeek JA
AD  - Research Unit Lung Toxicology, Katholieke Universiteit Leuven, B-3000 Leuven, 
      Belgium.
FAU - De Vooght, Vanessa
AU  - De Vooght V
FAU - Synhaeve, Nicholas
AU  - Synhaeve N
FAU - Nemery, Benoit
AU  - Nemery B
FAU - Hoet, Peter H M
AU  - Hoet PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090330
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins)
RN  - 0 (Phenylenediamines)
RN  - 0W5ETF9M2K (Methacholine Chloride)
RN  - 17X7AFZ1GH (Toluene 2,4-Diisocyanate)
RN  - IS1AKN4HYB (2,4-diaminotoluene)
SB  - IM
MH  - Animals
MH  - Antigens, CD/immunology
MH  - Asthma/*chemically induced/immunology
MH  - Cells, Cultured
MH  - Cross Reactions
MH  - Cytokines/immunology
MH  - Disease Models, Animal
MH  - Immunoglobulins/blood
MH  - Local Lymph Node Assay
MH  - Lymphocytes/immunology
MH  - Male
MH  - Methacholine Chloride/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phenylenediamines/administration & dosage/*immunology
MH  - Statistics, Nonparametric
MH  - Toluene 2,4-Diisocyanate/administration & dosage/*immunology
EDAT- 2009/04/01 09:00
MHDA- 2009/09/04 06:00
CRDT- 2009/04/01 09:00
PHST- 2009/04/01 09:00 [entrez]
PHST- 2009/04/01 09:00 [pubmed]
PHST- 2009/09/04 06:00 [medline]
AID - kfp065 [pii]
AID - 10.1093/toxsci/kfp065 [doi]
PST - ppublish
SO  - Toxicol Sci. 2009 Jun;109(2):256-64. doi: 10.1093/toxsci/kfp065. Epub 2009 Mar 
      30.