PMID- 19335447
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20161125
IS  - 1399-3054 (Electronic)
IS  - 0031-9317 (Linking)
VI  - 135
IP  - 4
DP  - 2009 Apr
TI  - In situ activities of hexokinase and fructokinase in relation to phosphorylation 
      status of root meristem cells of Vicia faba during reactivation from sugar 
      starvation.
PG  - 342-50
LID - 10.1111/j.1399-3054.2008.01201.x [doi]
AB  - The plant cell cycle is equipped with two principal control points: PCP1 in G1 
      and PCP2 in G2 phase. These checkpoints can arrest the cell cycle in response to 
      carbohydrate starvation, while sugar presence can revive the replication and 
      mitotic activity. The process of cell cycle revival is strongly repressed by 
      okadaic acid (OA) or 6-dimethylaminopurine (6-DMAP), inhibitors of specific 
      protein phosphatases 1 or 2A or kinases (cyclin-dependent kinases), respectively. 
      In the present study, it was investigated whether inhibition of cell cycle 
      revival is performed through interference of the above-mentioned inhibitors with 
      the metabolic pathway of sucrose applied to the cells. Changes of hexokinase (HK) 
      and fructokinase (FK) activities, key enzymes of hexose metabolism, were analyzed 
      in Vicia faba root meristem cells arrested in G1 and G2 phase by carbohydrate 
      starvation as well as in those recovered with glucose or sucrose in the presence 
      of OA or 6-DMAP. It was shown that in the sugar-starved cells, the activity of 
      both enzymes decreased significantly. During cell regeneration with 
      carbohydrates, the activity of HK was induced more by sucrose than by glucose, 
      while FK remained inactive after glucose addition. Moreover, in situ 
      investigation of the activities of HK and FK showed that OA-induced and 
      6-DMAP-induced repression of the cell cycle revival is connected with the 
      interference of these drugs in the metabolic pathway of sucrose. It was also 
      indicated that stronger OA-induced and 6-DMAP-induced inhibition of the 
      replication and mitosis revival, at the early stages of sucrose regeneration, was 
      correlated with the stronger influence of these inhibitors on HK and FK 
      activities.
FAU - Polit, Justyna T
AU  - Polit JT
AD  - Department of Cytophysiology, University of Lodz, Lodz, Poland. 
      justpoli@poczta.onet.pl
FAU - Ciereszko, Iwona
AU  - Ciereszko I
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Physiol Plant
JT  - Physiologia plantarum
JID - 1256322
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - 57-50-1 (Sucrose)
RN  - 649SA4S2CV (N(6),N(6)-dimethyladenine)
RN  - EC 2.7.1.- (Fructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.4 (fructokinase)
RN  - IY9XDZ35W2 (Glucose)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Carbohydrate Metabolism
MH  - Cell Cycle/drug effects
MH  - Fructokinases/*metabolism
MH  - Glucose/metabolism
MH  - Hexokinase/*metabolism
MH  - Meristem/cytology/*enzymology
MH  - Okadaic Acid/pharmacology
MH  - Phosphorylation
MH  - Sucrose/metabolism
MH  - Vicia faba/*enzymology
EDAT- 2009/04/02 09:00
MHDA- 2009/04/16 09:00
CRDT- 2009/04/02 09:00
PHST- 2009/04/02 09:00 [entrez]
PHST- 2009/04/02 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
AID - PPL1201 [pii]
AID - 10.1111/j.1399-3054.2008.01201.x [doi]
PST - ppublish
SO  - Physiol Plant. 2009 Apr;135(4):342-50. doi: 10.1111/j.1399-3054.2008.01201.x.