PMID- 19336532
OWN - NLM
STAT- MEDLINE
DCOM- 20100113
LR  - 20090811
IS  - 1531-2267 (Electronic)
IS  - 1094-8341 (Linking)
VI  - 38
IP  - 3
DP  - 2009 Aug 7
TI  - Gene expression profiles in peripheral blood mononuclear cells of chronic heart 
      failure patients.
PG  - 233-40
LID - 10.1152/physiolgenomics.90364.2008 [doi]
AB  - The present study was aimed at identifying chronic heart failure (CHF) biomarkers 
      from peripheral blood mononuclear cells (PBMCs) in patients with ischemic (ICM) 
      and nonischemic dilated (NIDCM) cardiomyopathy. PBMC gene expression profiling 
      was performed by Affymetrix in two patient groups, 1) ICM (n = 12) and 2) NIDCM 
      (n = 12) New York Heart Association (NYHA) III/IV CHF patients, vs. 3) age- and 
      sex-matched control subjects (n = 12). Extracted RNAs were then pooled and 
      hybridized to a total of 11 microarrays. Gene ontology (GO) analysis separated 
      gene profiling into functional classes. Prediction analysis of microarrays (PAM) 
      and significance analysis of microarrays (SAM) were utilized in order to identify 
      a molecular signature. Candidate markers were validated by quantitative real-time 
      polymerase chain reaction. We identified a gene expression profiling that 
      distinguished between CHF patients and control subjects. Interestingly, among the 
      set of genes constituting the signature, chemokine receptor (CCR2, CX(3)CR1) and 
      early growth response (EGR1, 2, 3) family members were found to be upregulated in 
      CHF patients vs. control subjects and to be part of a gene network. Such findings 
      were strengthened by the analysis of an additional 26 CHF patients (n = 14 ICM 
      and n = 12 NIDCM), which yielded similar results. The present study represents 
      the first large-scale gene expression analysis of CHF patient PBMCs that 
      identified a molecular signature of CHF and putative biomarkers of CHF, i.e., 
      chemokine receptor and EGR family members. Furthermore, EGR1 expression levels 
      can discriminate between ICM and NIDCM CHF patients.
FAU - Cappuzzello, Claudia
AU  - Cappuzzello C
AD  - Laboratorio di Patologia Vascolare, Istituto Dermopatico dell'Immacolata IRCCS, 
      Rome.
FAU - Napolitano, Monica
AU  - Napolitano M
FAU - Arcelli, Diego
AU  - Arcelli D
FAU - Melillo, Guido
AU  - Melillo G
FAU - Melchionna, Roberta
AU  - Melchionna R
FAU - Di Vito, Luca
AU  - Di Vito L
FAU - Carlini, Daniele
AU  - Carlini D
FAU - Silvestri, Lorena
AU  - Silvestri L
FAU - Brugaletta, Salvatore
AU  - Brugaletta S
FAU - Liuzzo, Giovanna
AU  - Liuzzo G
FAU - Crea, Filippo
AU  - Crea F
FAU - Capogrossi, Maurizio C
AU  - Capogrossi MC
LA  - eng
SI  - GEO/GSE1869
SI  - GEO/GSE3585
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090331
PL  - United States
TA  - Physiol Genomics
JT  - Physiological genomics
JID - 9815683
RN  - 0 (EGR1 protein, human)
RN  - 0 (Early Growth Response Protein 1)
SB  - IM
MH  - Aged
MH  - Blotting, Western
MH  - Chronic Disease
MH  - Cluster Analysis
MH  - Early Growth Response Protein 1/genetics/metabolism
MH  - Female
MH  - Gene Expression Profiling/*methods
MH  - Gene Regulatory Networks
MH  - Heart Failure/blood/*genetics/metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2009/04/02 09:00
MHDA- 2010/01/14 06:00
CRDT- 2009/04/02 09:00
PHST- 2009/04/02 09:00 [entrez]
PHST- 2009/04/02 09:00 [pubmed]
PHST- 2010/01/14 06:00 [medline]
AID - 90364.2008 [pii]
AID - 10.1152/physiolgenomics.90364.2008 [doi]
PST - ppublish
SO  - Physiol Genomics. 2009 Aug 7;38(3):233-40. doi: 
      10.1152/physiolgenomics.90364.2008. Epub 2009 Mar 31.