PMID- 1933827
OWN - NLM
STAT- MEDLINE
DCOM- 19911213
LR  - 20190815
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 55
IP  - 2
DP  - 1991 Sep
TI  - Interphase cytogenetics of a male breast cancer.
PG  - 243-7
AB  - Direct interphase cytogenetic analysis was performed on nuclei from a male breast 
      tumor using fluorescence in situ hybridization (FISH). DNA probes specific for 
      repetitive pericentromeric regions on chromosomes 1, 7, 9, 11, 15, 17, 18, X, and 
      Y were used to determine chromosome copy numbers in interphase tumor cells. Copy 
      number distributions varied greatly between chromosomes, showing major tumor 
      populations with on (Y), two (X,9), three (11, 15, 18), and four (1, 7, 17) 
      copies of the pericentromeric targets. The X chromosome was present in two copies 
      in 84.7% of tumor nuclei, with the balance being primarily monosomic. Normal skin 
      fibroblasts cultured from the same patient showed 99% monosomy X. The Y 
      chromosome showed a minor population (12%) with two copies. The DNA index of the 
      tumor was 2.0 as determined by flow cytometry. The proliferative activity of the 
      tumor cells was simultaneously analyzed using detection of in vivo 
      bromodeoxyuridine (BrdU) incorporation. The BrdU labeling index was 13.2%.
FAU - Balazs, M
AU  - Balazs M
AD  - Department of Laboratory Medicine, University of California San Francisco 
      94143-0808.
FAU - Mayall, B H
AU  - Mayall BH
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA 44768/CA/NCI NIH HHS/United States
GR  - CA 49056/CA/NCI NIH HHS/United States
GR  - CA45919/CA/NCI NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics
MH  - Bromodeoxyuridine/metabolism
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis/metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - *Interphase
MH  - Male
MH  - Nucleic Acid Hybridization
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 0165-4608(91)90084-8 [pii]
AID - 10.1016/0165-4608(91)90084-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1991 Sep;55(2):243-7. doi: 10.1016/0165-4608(91)90084-8.