PMID- 19339781
OWN - NLM
STAT- MEDLINE
DCOM- 20090806
LR  - 20131121
IS  - 1423-002X (Electronic)
IS  - 0378-7346 (Linking)
VI  - 67
IP  - 4
DP  - 2009
TI  - Effects of progesterone and its antagonist mifepristone on progesterone receptor 
      a expression in human umbilical vein endothelial cells.
PG  - 269-74
LID - 10.1159/000210373 [doi]
AB  - Effects of female steroid hormones on endothelial cells are gaining increased 
      importance due to several studies on the effects of hormonal treatment on 
      cardiovascular risk. Recent data argue for an improvement of endothelium-derived 
      relaxation and impaired vascular contraction by estradiol, whereas progesterone 
      and testosterone might entail contrary effects. So far, gestagenic influence on 
      endothelial cell physiology is poorly understood. Human umbilical vein 
      endothelial cells (HUVECs) exposed to the female sex hormones estradiol and 
      progesterone show expression of estrogen receptor-beta (ERbeta) and progesterone 
      receptor A (PR-A), and are negative for ERalpha and PR-B. The aim of this study 
      was to analyze the expression and stimulation of PR-A and -B in HUVECs after 
      stimulation with progesterone and PR antagonists that are commercially available. 
      PR-B expression or upregulation was abrogated after application of progesterone 
      or antagonists to HUVECs. Expression of PR-A could be significantly upregulated 
      with progesterone and mifepristone. Unexpectedly, stimulation with the 
      progesterone antagonist RU486 (mifepristone) was accomplished by an upregulation 
      of PR-A expression in our study. We conclude that gestagenic effects on HUVECs 
      independent of modulators are mediated via the PR-A.
CI  - Copyright (c) 2009 S. Karger AG, Basel.
FAU - Toth, Bettina
AU  - Toth B
AD  - Department of Obstetrics and Gynecology, Grosshadern, Germany.
FAU - Scholz, Christoph
AU  - Scholz C
FAU - Ochsenkuhn, Robert
AU  - Ochsenkuhn R
FAU - Schulze, Sandra
AU  - Schulze S
FAU - Kuhn, Christina
AU  - Kuhn C
FAU - Friese, Klaus
AU  - Friese K
FAU - Jeschke, Udo
AU  - Jeschke U
LA  - eng
PT  - Journal Article
DEP - 20090401
PL  - Switzerland
TA  - Gynecol Obstet Invest
JT  - Gynecologic and obstetric investigation
JID - 7900587
RN  - 0 (Receptors, Progesterone)
RN  - 0 (progesterone receptor A)
RN  - 320T6RNW1F (Mifepristone)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Cells, Cultured
MH  - Endothelial Cells/*chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Mifepristone/*pharmacology
MH  - Progesterone/antagonists & inhibitors/*pharmacology
MH  - Receptors, Progesterone/*analysis/drug effects
MH  - Umbilical Veins/*cytology
EDAT- 2009/04/03 09:00
MHDA- 2009/08/07 09:00
CRDT- 2009/04/03 09:00
PHST- 2008/08/07 00:00 [received]
PHST- 2008/12/09 00:00 [accepted]
PHST- 2009/04/03 09:00 [entrez]
PHST- 2009/04/03 09:00 [pubmed]
PHST- 2009/08/07 09:00 [medline]
AID - 000210373 [pii]
AID - 10.1159/000210373 [doi]
PST - ppublish
SO  - Gynecol Obstet Invest. 2009;67(4):269-74. doi: 10.1159/000210373. Epub 2009 Apr 
      1.