PMID- 19341334
OWN - NLM
STAT- MEDLINE
DCOM- 20090730
LR  - 20090403
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 26
IP  - 2
DP  - 2009 Feb
TI  - Quantitative study of apoptotic cells in the goldfish retina.
PG  - 157-62
LID - 10.2108/zsj.26.157 [doi]
AB  - In the vertebrate retina, it has been shown that a large number of neurons 
      produced at the onset of neurogenesis die early in development. Since this 
      apoptotic cell death occurs in a short, limited time, little is known in detail 
      on its histocytology. Using the terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) method, we investigated the apoptotic cells in the 
      adult goldfish retina in which the progenitor cell keeps proliferating. Most of 
      the TUNEL-positive nuclei were found in the marginal retina, about 40-200 microm 
      from the circular blood vessel (CBV) running parallel to the retinal rim. The 
      peak density was about 1400/mm(2) surface area at 90-130 microm from the CBV. 
      Overall retinal cell density in the marginal retina was also studied by toluidine 
      blue staining. The retinal cell density increased toward the central retina, 
      reaching a peak density of 278,000/mm(2)at 130 microm from the CBV. It then 
      decreased gradually to 228,000/mm(2) in the mature retina. This 18% reduction 
      seemed to be caused by apoptosis. We also found a significant increase in the 
      mean density of apoptotic cells in goldfish sacrificed at midnight, 403/mm(2), 
      compared to that at midday, 217/mm(2). This increase was not seen in goldfish 
      kept under either constant darkness or constant light.
FAU - Mizuno, Taka-aki
AU  - Mizuno TA
AD  - Laboratory of Neurobiology, Biology Research Division, Graduate School of 
      Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
FAU - Ohtsuka, Teruya
AU  - Ohtsuka T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Circadian Rhythm
MH  - Eye/blood supply
MH  - Goldfish/*anatomy & histology
MH  - Histocytochemistry
MH  - In Situ Nick-End Labeling
MH  - Neurogenesis
MH  - Neurons/*cytology
MH  - Retina/*cytology
EDAT- 2009/04/04 09:00
MHDA- 2009/07/31 09:00
CRDT- 2009/04/04 09:00
PHST- 2009/04/04 09:00 [entrez]
PHST- 2009/04/04 09:00 [pubmed]
PHST- 2009/07/31 09:00 [medline]
AID - 10.2108/zsj.26.157 [doi]
PST - ppublish
SO  - Zoolog Sci. 2009 Feb;26(2):157-62. doi: 10.2108/zsj.26.157.