PMID- 19342635
OWN - NLM
STAT- MEDLINE
DCOM- 20090423
LR  - 20211020
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 182
IP  - 8
DP  - 2009 Apr 15
TI  - Rabbit polyclonal mouse antithymocyte globulin administration alters dendritic 
      cell profile and function in NOD mice to suppress diabetogenic responses.
PG  - 4608-15
LID - 10.4049/jimmunol.0713269 [doi]
AB  - Mouse antithymocyte globulin (mATG) prevents, as well as reverses, type 1 
      diabetes in NOD mice, through mechanisms involving modulation of the 
      immunoregulatory activities of T lymphocytes. Dendritic cells (DC) play a pivotal 
      role in the generation of T cell responses, including those relevant to the 
      autoreactive T cells enabling type 1 diabetes. As Abs against DC are likely 
      generated during production of mATG, we examined the impact of this preparation 
      on the phenotype and function of DC to elucidate novel mechanisms underlying its 
      beneficial activities. In vivo, mATG treatment transiently induced the 
      trafficking of mature CD8(-) predominant DC into the pancreatic lymph node of NOD 
      mice. Splenic DC from mATG-treated mice also exhibited a more mature phenotype 
      characterized by reduced CD8 expression and increased IL-10 production. The 
      resultant DC possessed a potent capacity to induce Th2 responses when cultured ex 
      vivo with diabetogenic CD4(+) T cells obtained from BDC2.5 TCR transgenic mice. 
      Cotransfer of these Th2-deviated CD4(+) T cells with splenic cells from newly 
      diabetic NOD mice into NOD.RAG(-/-) mice significantly delayed the onset of 
      diabetes. These studies suggest the alteration of DC profile and function by mATG 
      may skew the Th1/Th2 balance in vivo and through such actions, represent an 
      additional novel mechanism by which this agent provides its beneficial 
      activities.
FAU - Huang, Yanfei
AU  - Huang Y
AD  - Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21204, USA.
FAU - Parker, Matthew
AU  - Parker M
FAU - Xia, Changqing
AU  - Xia C
FAU - Peng, Ruihua
AU  - Peng R
FAU - Wasserfall, Clive
AU  - Wasserfall C
FAU - Clarke, Tracy
AU  - Clarke T
FAU - Wu, Lizhen
AU  - Wu L
FAU - Chowdhry, Tayseer
AU  - Chowdhry T
FAU - Campbell-Thompson, Martha
AU  - Campbell-Thompson M
FAU - Williams, John
AU  - Williams J
FAU - Clare-Salzler, Michael
AU  - Clare-Salzler M
FAU - Atkinson, Mark A
AU  - Atkinson MA
FAU - Womer, Karl L
AU  - Womer KL
LA  - eng
GR  - K08DK66319/DK/NIDDK NIH HHS/United States
GR  - K08 DK066319-04/DK/NIDDK NIH HHS/United States
GR  - DK063422/DK/NIDDK NIH HHS/United States
GR  - K08 DK066319-03/DK/NIDDK NIH HHS/United States
GR  - R33 DK063422/DK/NIDDK NIH HHS/United States
GR  - K08 DK066319/DK/NIDDK NIH HHS/United States
GR  - 394288/PHS HHS/United States
GR  - P01 AI042288/AI/NIAID NIH HHS/United States
GR  - 394210/PHS HHS/United States
GR  - K08 DK066319-05/DK/NIDDK NIH HHS/United States
GR  - P01 AI042288-11/AI/NIAID NIH HHS/United States
GR  - N01 DK063422/DK/NIDDK NIH HHS/United States
GR  - R21 DK063422/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antilymphocyte Serum)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Antilymphocyte Serum/*administration & dosage/*immunology/pharmacology
MH  - Bone Marrow/immunology
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/cytology/*drug effects/*immunology/metabolism
MH  - Diabetes Mellitus/*immunology
MH  - Female
MH  - Interleukin-10/biosynthesis/immunology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Rabbits
MH  - Spleen/immunology
MH  - Th2 Cells/immunology
PMC - PMC2770841
MID - NIHMS108443
EDAT- 2009/04/04 09:00
MHDA- 2009/04/25 09:00
PMCR- 2010/04/15
CRDT- 2009/04/04 09:00
PHST- 2009/04/04 09:00 [entrez]
PHST- 2009/04/04 09:00 [pubmed]
PHST- 2009/04/25 09:00 [medline]
PHST- 2010/04/15 00:00 [pmc-release]
AID - 182/8/4608 [pii]
AID - 10.4049/jimmunol.0713269 [doi]
PST - ppublish
SO  - J Immunol. 2009 Apr 15;182(8):4608-15. doi: 10.4049/jimmunol.0713269.