PMID- 19344657
OWN - NLM
STAT- MEDLINE
DCOM- 20090623
LR  - 20090420
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 387
IP  - 2
DP  - 2009 Apr 15
TI  - Multiplex genotyping and allele frequency estimation in pooled DNAs using non-gel 
      capillary electrophoresis.
PG  - 221-9
LID - 10.1016/j.ab.2009.01.021 [doi]
AB  - We present a novel approach of single-nucleotide polymorphism (SNP) analysis in 
      which allele-specific oligonucleotide hybridization is followed by non-gel 
      capillary electrophoresis (ASOH-NGCE) in conjunction with laser-induced 
      fluorescence (LIF). This allows rapid multiplex allelotyping and allele frequency 
      estimation. This method, based on site separation of the hybridization duplexes, 
      retains the simplicity and specificity of ASOH and the homogeneous feature of 
      NGCE with poly(N,N-dimethylacrylamide) (PDMA) as a sieving medium. ASOH-NGCE can 
      be applied to multiplex SNP loci genotyping with excellent separation of 
      hybridization mixtures. Average relative standard deviations (RSDs) were low for 
      within-day (1.10%) and between-day (2.41%) reproducibility. Moreover, the allele 
      frequencies in pooled DNAs were accurately determined from peak areas and 
      equilibrium dissociation constants. Our method was highly sensitive in detecting 
      alleles with frequency as low as 1% and in distinguishing allele frequencies 
      differing by 1% between pools. The average value of differences between real and 
      estimated frequencies (accuracy) was only 0.004.
FAU - Yin, Bin-Cheng
AU  - Yin BC
AD  - Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor 
      Engineering, East China University of Science and Technology, Shanghai 200237, 
      China.
FAU - Wang, Xian-Fei
AU  - Wang XF
FAU - Ye, Bang-Ce
AU  - Ye BC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090121
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - *Gene Frequency
MH  - Genotype
MH  - *Polymorphism, Single Nucleotide
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2009/04/07 09:00
MHDA- 2009/06/24 09:00
CRDT- 2009/04/07 09:00
PHST- 2008/11/06 00:00 [received]
PHST- 2009/01/14 00:00 [revised]
PHST- 2009/01/15 00:00 [accepted]
PHST- 2009/04/07 09:00 [entrez]
PHST- 2009/04/07 09:00 [pubmed]
PHST- 2009/06/24 09:00 [medline]
AID - S0003-2697(09)00050-5 [pii]
AID - 10.1016/j.ab.2009.01.021 [doi]
PST - ppublish
SO  - Anal Biochem. 2009 Apr 15;387(2):221-9. doi: 10.1016/j.ab.2009.01.021. Epub 2009 
      Jan 21.