PMID- 19344696 OWN - NLM STAT- MEDLINE DCOM- 20090807 LR - 20131121 IS - 1872-6240 (Electronic) IS - 0006-8993 (Linking) VI - 1272 DP - 2009 May 26 TI - Brain-derived neurotrophic factor and electrophysiological properties of voltage-gated ion channels during neuronal stem cell development. PG - 14-24 LID - 10.1016/j.brainres.2009.03.048 [doi] AB - Brain-derived neurotrophic factor (BDNF) has been reported to play a critical role in modulating a variety of neural functions such as membrane excitability, synaptic transmission, and activity-dependent synaptic plasticity. BDNF has also been found to enhance neurogenesis. Although consensus is found on the general trophic effected by BDNF on subpopulations of neurons, little information appears concerning the effects of BDNF on ion currents during the development of these newborn cells. Here, we report that BDNF plays an essential role in the development of neural stem cells during a plastic period in vitro. We found that chronic stimulation of neural stem cells with bath application of 40 ng/ml BDNF during differentiation promoted the functional development of passive membrane, evoked biphasic changes in Na(+) currents, regulated the expression of K(+) channels and the outward K(+) especially at the early developmental stage. In conclusion, our findings indicate that BDNF principally regulates the electrical properties of neural stem cell (NSC) especially in the early developmental stage. FAU - Leng, Jie AU - Leng J AD - Department of Neurology, Children's Hospital of Chongqing Medical University, 136# Zhongshan 2 Road, Chongqing, 400014, People's Republic of China. FAU - Jiang, Li AU - Jiang L FAU - Chen, Hengsheng AU - Chen H FAU - Zhang, Xiaoping AU - Zhang X LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090401 PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (Ion Channels) RN - 0 (Potassium Channel Blockers) RN - 0 (Sodium Channel Blockers) RN - 0 (Tubulin) RN - 4368-28-9 (Tetrodotoxin) RN - 66-40-0 (Tetraethylammonium) RN - BH3B64OKL9 (4-Aminopyridine) SB - IM MH - 4-Aminopyridine/pharmacology MH - Analysis of Variance MH - Animals MH - Biophysical Phenomena/drug effects MH - Biophysics MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cell Differentiation/*drug effects MH - Cells, Cultured MH - Electric Stimulation MH - Embryo, Mammalian MH - Embryonic Stem Cells/*physiology MH - Glial Fibrillary Acidic Protein/metabolism MH - Hippocampus/cytology MH - Humans MH - Ion Channel Gating/*drug effects MH - Ion Channels/*physiology MH - Membrane Potentials/drug effects/physiology MH - Patch-Clamp Techniques/methods MH - Potassium Channel Blockers/pharmacology MH - Rats MH - Sodium Channel Blockers/pharmacology MH - Tetraethylammonium/pharmacology MH - Tetrodotoxin/pharmacology MH - Tubulin/metabolism EDAT- 2009/04/07 09:00 MHDA- 2009/08/08 09:00 CRDT- 2009/04/07 09:00 PHST- 2009/02/13 00:00 [received] PHST- 2009/03/14 00:00 [revised] PHST- 2009/03/17 00:00 [accepted] PHST- 2009/04/07 09:00 [entrez] PHST- 2009/04/07 09:00 [pubmed] PHST- 2009/08/08 09:00 [medline] AID - S0006-8993(09)00607-6 [pii] AID - 10.1016/j.brainres.2009.03.048 [doi] PST - ppublish SO - Brain Res. 2009 May 26;1272:14-24. doi: 10.1016/j.brainres.2009.03.048. Epub 2009 Apr 1.