PMID- 19345144 OWN - NLM STAT- MEDLINE DCOM- 20091015 LR - 20191210 IS - 1618-0607 (Electronic) IS - 1438-4221 (Linking) VI - 299 IP - 6 DP - 2009 Aug TI - Rapid identification of beta-hemolytic streptococci by fluorescence in situ hybridization (FISH). PG - 421-6 LID - 10.1016/j.ijmm.2009.02.001 [doi] AB - Rapid identification of pathogenic, beta-hemolytic streptococci is important for treatment decisions. We evaluated fluorescence in situ hybridization (FISH) for this purpose using 23 reference strains, 157 clinical isolates, and 80 blood cultures showing streptococci in the Gram stain. With a sensitivity and specificity in excess of 99%, FISH proved to be suitable for rapid identification of beta-hemolytic streptococci in a diagnostic laboratory. FAU - Poppert, Sven AU - Poppert S AD - Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany. poppert@bni-hamburg.de FAU - Nickel, Daniel AU - Nickel D FAU - Berger, Anja AU - Berger A FAU - Yildiz, Tatjana AU - Yildiz T FAU - Kaestner, Nicole AU - Kaestner N FAU - Mauerer, Stefanie AU - Mauerer S FAU - Spellerberg, Barbara AU - Spellerberg B LA - eng PT - Evaluation Study PT - Journal Article DEP - 20090402 PL - Germany TA - Int J Med Microbiol JT - International journal of medical microbiology : IJMM JID - 100898849 RN - 0 (DNA-Binding Proteins) RN - 0 (beta protein, Streptococcus) SB - IM MH - DNA-Binding Proteins MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - Sensitivity and Specificity MH - Streptococcal Infections/*diagnosis/microbiology MH - Streptococcus pyogenes/genetics/*isolation & purification EDAT- 2009/04/07 09:00 MHDA- 2009/10/16 06:00 CRDT- 2009/04/07 09:00 PHST- 2008/11/25 00:00 [received] PHST- 2009/02/03 00:00 [revised] PHST- 2009/02/08 00:00 [accepted] PHST- 2009/04/07 09:00 [entrez] PHST- 2009/04/07 09:00 [pubmed] PHST- 2009/10/16 06:00 [medline] AID - S1438-4221(09)00019-8 [pii] AID - 10.1016/j.ijmm.2009.02.001 [doi] PST - ppublish SO - Int J Med Microbiol. 2009 Aug;299(6):421-6. doi: 10.1016/j.ijmm.2009.02.001. Epub 2009 Apr 2.