PMID- 19346484
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 106
IP  - 16
DP  - 2009 Apr 21
TI  - Switching direction in electric-signal-induced cell migration by cyclic guanosine 
      monophosphate and phosphatidylinositol signaling.
PG  - 6667-72
LID - 10.1073/pnas.0809974106 [doi]
AB  - Switching between attractive and repulsive migration in cell movement in response 
      to extracellular guidance cues has been found in various cell types and is an 
      important cellular function for translocation during cellular and developmental 
      processes. Here we show that the preferential direction of migration during 
      electrotaxis in Dictyostelium cells can be reversed by genetically modulating 
      both guanylyl cyclases (GCases) and the cyclic guanosine monophosphate 
      (cGMP)-binding protein C (GbpC) in combination with the inhibition of 
      phosphatidylinositol-3-OH kinases (PI3Ks). The PI3K-dependent pathway is involved 
      in cathode-directed migration under a direct-current electric field. The 
      catalytic domains of soluble GCase (sGC) and GbpC also mediate cathode-directed 
      signaling via cGMP, whereas the N-terminal domain of sGC mediates anode-directed 
      signaling in conjunction with both the inhibition of PI3Ks and cGMP production. 
      These observations provide an identification of the genes required for 
      directional switching in electrotaxis and suggest that a parallel processing of 
      electric signals, in which multiple-signaling pathways act to bias cell movement 
      toward the cathode or anode, is used to determine the direction of migration.
FAU - Sato, Masayuki J
AU  - Sato MJ
AD  - Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka 
      University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Kuwayama, Hidekazu
AU  - Kuwayama H
FAU - van Egmond, Wouter N
AU  - van Egmond WN
FAU - Takayama, Airi L K
AU  - Takayama AL
FAU - Takagi, Hiroaki
AU  - Takagi H
FAU - van Haastert, Peter J M
AU  - van Haastert PJ
FAU - Yanagida, Toshio
AU  - Yanagida T
FAU - Ueda, Masahiro
AU  - Ueda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090403
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Cell Movement/*drug effects
MH  - Cyclic GMP/*pharmacology
MH  - Dictyostelium/*cytology/*drug effects/enzymology
MH  - *Electricity
MH  - Enzyme Inhibitors/pharmacology
MH  - Guanylate Cyclase/antagonists & inhibitors/chemistry
MH  - Intracellular Space/drug effects/metabolism
MH  - Models, Biological
MH  - Mutation/genetics
MH  - Phosphatidylinositols/*pharmacology
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Structure, Tertiary
MH  - Protein Transport/drug effects
MH  - Signal Transduction/*drug effects
PMC - PMC2672521
COIS- The authors declare no conflict of interest.
EDAT- 2009/04/07 09:00
MHDA- 2009/05/30 09:00
PMCR- 2009/10/21
CRDT- 2009/04/07 09:00
PHST- 2009/04/07 09:00 [entrez]
PHST- 2009/04/07 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
PHST- 2009/10/21 00:00 [pmc-release]
AID - 0809974106 [pii]
AID - 7521 [pii]
AID - 10.1073/pnas.0809974106 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6667-72. doi: 
      10.1073/pnas.0809974106. Epub 2009 Apr 3.