PMID- 19346487
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 106
IP  - 16
DP  - 2009 Apr 21
TI  - Antigen storage compartments in mature dendritic cells facilitate prolonged 
      cytotoxic T lymphocyte cross-priming capacity.
PG  - 6730-5
LID - 10.1073/pnas.0900969106 [doi]
AB  - Dendritic cells (DCs) are crucial for priming of naive CD8(+) T lymphocytes to 
      exogenous antigens, so-called "cross-priming." We report that exogenous protein 
      antigen can be conserved for several days in mature DCs, coinciding with strong 
      cytotoxic T lymphocyte cross-priming potency in vivo. After MHC class I peptide 
      elution, protein antigen-derived peptide presentation is efficiently restored, 
      indicating the presence of an intracellular antigen depot. We characterized this 
      depot as a lysosome-like organelle, distinct from MHC class II compartments and 
      recently described early endosomal compartments that allow acute antigen 
      presentation in MHC class I. The storage compartments we report here facilitate 
      continuous supply of MHC class I ligands. This mechanism ensures sustained 
      cross-presentation by DCs, despite the short-lived expression of MHC class 
      I-peptide complexes at the cell surface.
FAU - van Montfoort, Nadine
AU  - van Montfoort N
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
FAU - Camps, Marcel G
AU  - Camps MG
FAU - Khan, Selina
AU  - Khan S
FAU - Filippov, Dmitri V
AU  - Filippov DV
FAU - Weterings, Jimmy J
AU  - Weterings JJ
FAU - Griffith, Janice M
AU  - Griffith JM
FAU - Geuze, Hans J
AU  - Geuze HJ
FAU - van Hall, Thorbald
AU  - van Hall T
FAU - Verbeek, J Sjef
AU  - Verbeek JS
FAU - Melief, Cornelis J
AU  - Melief CJ
FAU - Ossendorp, Ferry
AU  - Ossendorp F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090403
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigens/*immunology
MH  - Cell Compartmentation/*immunology
MH  - Cell Differentiation/*immunology
MH  - Cell Membrane/metabolism
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/*cytology/*immunology/ultrastructure
MH  - Histocompatibility Antigens Class I/immunology
MH  - Intracellular Space/metabolism
MH  - Lysosomes/metabolism/ultrastructure
MH  - Mice
MH  - Peptides/immunology
MH  - Protein Stability
MH  - Receptors, Immunologic/metabolism
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Time Factors
PMC - PMC2672553
COIS- The authors declare no conflict of interest.
EDAT- 2009/04/07 09:00
MHDA- 2009/05/30 09:00
PMCR- 2009/10/21
CRDT- 2009/04/07 09:00
PHST- 2009/04/07 09:00 [entrez]
PHST- 2009/04/07 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
PHST- 2009/10/21 00:00 [pmc-release]
AID - 0900969106 [pii]
AID - 7419 [pii]
AID - 10.1073/pnas.0900969106 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6730-5. doi: 
      10.1073/pnas.0900969106. Epub 2009 Apr 3.