PMID- 19347051
OWN - NLM
STAT- MEDLINE
DCOM- 20090622
LR  - 20211020
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 15
DP  - 2009
TI  - Involvement of brain-derived neurotrophic factor in time-dependent 
      neurodegeneration in the murine superior colliculus after intravitreal injection 
      of N-methyl-D-aspartate.
PG  - 662-9
AB  - PURPOSE: To clarify the effects on the visual pathway that occur following 
      retinal damage, we examined the morphological alterations present in the superior 
      colliculus (SC) after N-methyl-D-aspartate (NMDA)-induced retinal damage in mice. 
      METHODS: NMDA was injected into the vitreous body of the left eye in mice to 
      induce retinal damage. The time-dependent neuronal degeneration in the SC was 
      assessed using immunohistochemistry. RESULTS: The number of neuronal nuclear 
      specific protein (NeuN)-immunostained neurons showed a significant decrease in 
      the contralateral SC at both 90 and 180 days after intravitreal NMDA injection. 
      In contrast, the ipsilateral SC displayed no significant change in the number of 
      NeuN-positive cells. An increase in glial fibrillary acid protein (GFAP) 
      immunoreactivity was observed in the contralateral SC at 7, 30, and 90 days after 
      NMDA injection and in the ipsilateral SC at 7 days, while brain-derived 
      neurotrophic factor (BDNF) expression was increased in the contralateral SC at 30 
      and 90 days. In the contralateral SC, some GFAP-positive astroglial cells also 
      exhibited BDNF at 30 days after NMDA injection. CONCLUSIONS: Evidence of 
      time-dependent morphological neuronal degeneration along the retinocollicular 
      pathway from the retina to the SC was detected at 90 and 180 days, but not at 30 
      days, after NMDA-induced retinal damage. This neurodegeneration was preceded by 
      an increase in BDNF expression in the SC, specifically at 30 and 90 days after 
      NMDA injection. Hence, these findings may provide useful information concerning 
      the pathological mechanisms of several disorders accompanied by retinal 
      degeneration.
FAU - Tanaka, Hirotaka
AU  - Tanaka H
AD  - 1Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu 
      Pharmaceutical University, Gifu, Japan.
FAU - Ito, Yasushi
AU  - Ito Y
FAU - Nakamura, Shinsuke
AU  - Nakamura S
FAU - Shimazawa, Masamitsu
AU  - Shimazawa M
FAU - Hara, Hideaki
AU  - Hara H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090403
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (NeuN protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Animals
MH  - Astrocytes/drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - DNA-Binding Proteins
MH  - Drug Administration Routes
MH  - Fluorescent Antibody Technique
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Injections
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - N-Methylaspartate/*administration & dosage/*pharmacology
MH  - Nerve Degeneration/*metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/drug effects/metabolism/pathology
MH  - Nuclear Proteins/metabolism
MH  - Retina/drug effects/metabolism
MH  - Superior Colliculi/drug effects/*metabolism/*pathology
MH  - Time Factors
PMC - PMC2664844
EDAT- 2009/04/07 09:00
MHDA- 2009/06/23 09:00
PMCR- 2009/01/01
CRDT- 2009/04/07 09:00
PHST- 2008/12/03 00:00 [received]
PHST- 2009/03/21 00:00 [accepted]
PHST- 2009/04/07 09:00 [entrez]
PHST- 2009/04/07 09:00 [pubmed]
PHST- 2009/06/23 09:00 [medline]
PHST- 2009/01/01 00:00 [pmc-release]
AID - 68 [pii]
AID - 2008MOLVIS0389 [pii]
PST - ppublish
SO  - Mol Vis. 2009;15:662-9. Epub 2009 Apr 3.