PMID- 19347312
OWN - NLM
STAT- MEDLINE
DCOM- 20090520
LR  - 20171116
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 531
DP  - 2009
TI  - Probing in vivo origins of mononuclear phagocytes by conditional ablation and 
      reconstitution.
PG  - 71-87
LID - 10.1007/978-1-59745-396-7_6 [doi]
AB  - Dendritic cells (DCs) and macrophages (MPhis), collectively termed mononuclear 
      phagocytes (MP), are crucial for homeostatic tissue maintenance as well as the 
      innate and adaptive host defense. These pleiotropic functions are likely to be 
      covered by distinct DC and MPhi subsets, defined by anatomic location and 
      molecular make-up. However, task division within the MP system remains poorly 
      defined. A key to understanding of this issue, which might have important 
      implications for the development of future therapeutic strategies, is the 
      elucidation of the in vivo origins of DCs and MPhis, whose study recently gained 
      striking momentum. Here we present methods to investigate the role of MP 
      progenitors, such as monocytes and MPhi/DC precursors (MDPs), in the 
      replenishment of the peripheral MP system.
FAU - Varol, Chen
AU  - Varol C
AD  - Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
FAU - Landsman, Limor
AU  - Landsman L
FAU - Jung, Steffen
AU  - Jung S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (CD11c Antigen)
RN  - 0 (HBEGF protein, human)
RN  - 0 (Hbegf protein, mouse)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Ablation Techniques/*methods
MH  - Adoptive Transfer
MH  - Animals
MH  - CD11c Antigen/metabolism
MH  - Heparin-binding EGF-like Growth Factor
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Leukocytes, Mononuclear/*cytology/transplantation
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/cytology
MH  - Phagocytes/*cytology/transplantation
EDAT- 2009/04/07 09:00
MHDA- 2009/05/21 09:00
CRDT- 2009/04/07 09:00
PHST- 2009/04/07 09:00 [entrez]
PHST- 2009/04/07 09:00 [pubmed]
PHST- 2009/05/21 09:00 [medline]
AID - 10.1007/978-1-59745-396-7_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;531:71-87. doi: 10.1007/978-1-59745-396-7_6.