PMID- 19347831
OWN - NLM
STAT- MEDLINE
DCOM- 20090430
LR  - 20220408
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 117
IP  - 1
DP  - 2009 Feb 25
TI  - Loss of heterozygosities in Barrett esophagus, dysplasia, and adenocarcinoma 
      detected by esophageal brushing cytology and gastroesophageal biopsy.
PG  - 57-66
LID - 10.1002/cncy.20010 [doi]
AB  - BACKGROUND: Esophageal brushing cytology (EBC) and gastroesophageal biopsy (GEB) 
      are complementary procedures for the evaluation of gastroesophageal lesions that 
      help guide surveillance and treatment. METHODS: The authors investigated loss of 
      heterozygosity (LOH) of 17 microsatellite repeat markers near tumor suppressor 
      genes in gastroesophageal lesions on 34 concomitant EBCs and GEBs. RESULTS: The 
      results indicated that there was progressive accumulation of LOHs toward 
      malignant transformation. EBC samples a greater area than GEB, and more LOHs are 
      detected by EBC than GEB. The combination of cytomorphology and detection of LOHs 
      can improve diagnostic accuracy and is a more useful methodology with which to 
      evaluate gastroesophageal lesions than either EBC or GEB alone. The authors also 
      found that LOHs at 1p36, 9p21, and 17p13 may play an important role in Barrett 
      esophagus (BE), LOHs at 10q23, 17p13, and 17q12 in low-grade dysplasia (LGD), 
      LOHs at 5q23 and 17q21 in high-grade dysplasia (HGD), and LOHs at 5q23 and 21q22 
      in adenocarcinoma. CONCLUSIONS: Detection of LOHs targeting tumor suppressor 
      genes can be useful in evaluating gastroesophageal lesions, studying oncogenesis 
      of gastroesophageal adenocarcinoma, and, in combination with EBC and GEB, 
      determining surveillance for BE and LGD and/or treatment for HGD and 
      adenocarcinoma.
CI  - (c) 2009 American Cancer Society.
FAU - Lin, Xiaoqi
AU  - Lin X
AD  - Department of Pathology, Northwestern University, 251 East Huron Street, Chicago, 
      IL 60611, USA. xlin@northwestern.edu
FAU - Finkelstein, Sydney D
AU  - Finkelstein SD
FAU - Zhu, Bing
AU  - Zhu B
FAU - Ujevich, Beth J
AU  - Ujevich BJ
FAU - Silverman, Jan F
AU  - Silverman JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adenocarcinoma/diagnosis/*genetics/surgery
MH  - Barrett Esophagus/diagnosis/*genetics/surgery
MH  - Biopsy
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Cytodiagnosis/*methods
MH  - Cytological Techniques/methods
MH  - Esophageal Neoplasms/diagnosis/*genetics/surgery
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Microsatellite Repeats
MH  - Polymerase Chain Reaction
MH  - Precancerous Conditions/diagnosis/*genetics/surgery
EDAT- 2009/04/07 09:00
MHDA- 2009/05/01 09:00
CRDT- 2009/04/07 09:00
PHST- 2009/04/07 09:00 [entrez]
PHST- 2009/04/07 09:00 [pubmed]
PHST- 2009/05/01 09:00 [medline]
AID - 10.1002/cncy.20010 [doi]
PST - ppublish
SO  - Cancer. 2009 Feb 25;117(1):57-66. doi: 10.1002/cncy.20010.