PMID- 19349127
OWN - NLM
STAT- MEDLINE
DCOM- 20091026
LR  - 20130131
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 138
IP  - 1-2
DP  - 2009 Jul 2
TI  - Genetic variation analysis of porcine reproductive and respiratory syndrome virus 
      isolated in China from 2002 to 2007 based on ORF5.
PG  - 150-5
LID - 10.1016/j.vetmic.2009.03.001 [doi]
AB  - The complete open reading frame 5 (ORF5) sequences of 34 field porcine 
      reproductive and respiratory syndrome virus (PRRSV) isolates from China in 
      2002-2007 were detected and compared with the different variable Chinese isolates 
      S1, CH-1a, HB-1, HB-2 and JXA1. The results showed that all isolates were of type 
      2 PRRSV and could be assigned to two clusters. The isolates in cluster sg1 was 
      high similar with the highly pathogenic PRRSV strain JXA1, while sg2 clustered 
      with type 2 PRRSV isolate VR2332. It was interesting that the isolate SH02 which 
      was isolated from Shanghai in 2002 has 98.8% identity with JXA1 emerged in 2006. 
      And the ZJJ07 isolate was found to be a natural recombinant between a Chinese 
      highly pathogenic SY0608 isolate and a VR-2332 derivative NH04 isolate. Analysis 
      of the potential glycosylation sites indicated that they were frequently mutated 
      and formed five putative N-linked glycosylation (NGS) sites patterns based on 
      N30, 33-35, 44 and 51 in those isolates. It indicated that the highly variable 
      PRRSV strain with different NGS patterns spread widely in China. The great 
      genetic diversity could be taken into consideration for the control and 
      prevention of this disease.
FAU - Li, Yufeng
AU  - Li Y
AD  - Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, China.
FAU - Wang, Xinglong
AU  - Wang X
FAU - Jiang, Ping
AU  - Jiang P
FAU - Wang, Xiaofeng
AU  - Wang X
FAU - Chen, Wen
AU  - Chen W
FAU - Wang, Xianwei
AU  - Wang X
FAU - Wang, Kewei
AU  - Wang K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090313
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - China
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - *Genetic Variation
MH  - Geography
MH  - Glycosylation
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/isolation & purification
MH  - RNA, Viral/genetics/isolation & purification
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Viral Envelope Proteins/genetics
MH  - Viral Proteins/*genetics
EDAT- 2009/04/08 09:00
MHDA- 2009/10/27 06:00
CRDT- 2009/04/08 09:00
PHST- 2008/06/24 00:00 [received]
PHST- 2009/02/10 00:00 [revised]
PHST- 2009/03/02 00:00 [accepted]
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/10/27 06:00 [medline]
AID - S0378-1135(09)00105-9 [pii]
AID - 10.1016/j.vetmic.2009.03.001 [doi]
PST - ppublish
SO  - Vet Microbiol. 2009 Jul 2;138(1-2):150-5. doi: 10.1016/j.vetmic.2009.03.001. Epub 
      2009 Mar 13.